脑梗死患者血清中异常表达的microRNA-1298及其临床诊断价值分析

Abnormal Expression of microRNA-1298 in Serum of Patients with Acute Cerebral Infarction and Its Clinical Diagnostic Value

摘要: 目的：急性脑梗死(acute cerebral infarction, ACI)是一种高发病率、高致残率的脑部疾病。本研究的目的是探讨miR-1298在ACI患者中的临床价值。方法：使用qRT-PCR测定对照组和ACI组的miR-1298的相对表达水平。MiR-1298的临床预测价值通过绘制受试者工作特征(receiver operating charac-teristic, ROC)曲线进行评估。临床指标和ACI患者临床预后的相关性通过Logistic回归分析进行评价。MiR-1298的靶基因通过双荧光素酶报告实验进行验证。结果：MiR-1298在ACI患者中表达降低，随着病情的严重和梗死面积的增大，其表达水平进一步降低。MiR-1298可能成为ACI的诊断因子之一。MiR-1298在ACI预后不良组的表达量降低，并且可以独立预测ACI的不良预后。SETD7是miR-1298的靶基因，在ACI患者中表达量增加。结论：低表达的miR-1298可以作为ACI的诊断因子，并且可以预测ACI不良结局。

Abstract: Purpose: Acute cerebral infarction (ACI) is a brain disease with high morbidity and disability rate. The purpose of this study is to explore the clinical value of miR-1298 in patients with ACI. Methods: The relative expression level of miR-1298 in the control group and ACI group was determined by qRT-PCR. The clinical predictive value of miR-1298 was evaluated by drawing the receiver operat-ing characteristic (ROC) curve. The correlation between clinical indexes and the clinical prognosis of ACI patients was evaluated by Logistic regression analysis. The target gene of miR-1298 was veri-fied by a double luciferase report experiment. Results: The expression of miR-1298 decreased in ACI patients, and its expression level further decreased with the severity of the disease and the in-crease of infarct area. MiR-1298 might be one of the diagnostic factors of ACI. The expression of MiR-1298 decreased in the group with a poor prognosis of ACI, and it could independently predict the poor prognosis of ACI. SETD7 was the target gene of miR-1298, and its expression level was in-creased in ACI patients. Conclusion: MiR-1298 with low expression could be used as a diagnostic factor of ACI and predict the adverse outcome of ACI.