基于碳化聚合物点的荧光量子产率创新实验设计

Innovative Experimental Design of Fluorescence Quantum Yield Based on Carbonized Polymer Dots

摘要: 本研究将碳化聚合物点的最新科研成果转化为实验教学内容，设计了“基于碳化聚合物点的荧光量子产率创新实验”。具体实验以聚乙烯亚胺为原料，通过“自下而上”一步水热法合成了一种具有高荧光量子产率的碳化聚合物点(CPDs)，利用透射电子显微镜、荧光分光光度计和紫外分光光度计进行表征。同时考察了“一点法”和“比较法”两种方法对CPDs荧光量子产率的测定，并得出“比较法”测定更为准确的结论。本实验的开展，有利于加深学生对荧光产生机制的理解，深化对仪器组成部件的认知，提高实验数据处理、分析解决问题的能力，切实改善荧光分析实验教学的质量。

Abstract: In this study, the latest scientific research results of carbonized polymer dots were transformed into experimental teaching content, and the “Innovative experiment of fluorescence quantum Yield based on carbonized polymer dots” was designed. Carbonized polymer dots (CPDs) with high fluo-rescence quantum yield were synthesized from polyethyleneimine by bottom-up hydrothermal method. They were characterized by transmission electron microscopy, fluorescence spectropho-tometer and ultraviolet spectrophotometer. At the same time, the determination of fluorescence quantum yield of CPDs by “one point method” and “comparison method” is investigated, and the conclusion that “comparison method” is more accurate is obtained. The implementation of this experiment is conducive to deepening students’ understanding of the mechanism of fluorescence generation, deepening their cognition of the components of the instrument, improving the ability of experimental data processing, analysis and problem solving, and effectively improving the quality of fluorescence analysis experiment teaching.