广西崇左野生猕猴粪便皮质醇水平季节差异比较

Seasonal Variation in Fecal Cortisol Levels of Rhesus Macaques Living in Chongzuo, Guangxi

摘要: 动物的生理激素研究对监测其健康具有重要意义，皮质醇水平表征动物对环境的应激压力水平。本研究采用酶联免疫法对崇左野生猕猴(Macaca mulatta)粪便皮质醇含量进行测定，并分析不同季节激素含量差异。结果表明：崇左猕猴粪便皮质醇含量在5.02~10.03 μg/g之间，平均值为7.50 ± 1.77 μg/g。雨季崇左野生猕猴粪便皮质醇平均含量为9.04 ± 0.99 μg/g，旱季野生猕猴群粪便皮质醇平均含量为6.84 ± 1.55 μg/g。分析发现，崇左野生猕猴粪便皮质醇含量在季节上存在显著差异，表现为雨季皮质醇含量显著高于旱季皮质醇的含量(t = 2.969, df = 18, P = 0.008)。本研究评估了石山生境猕猴的应激状态，为其提供了重要的生理数据信息和健康监测新视角。

Abstract: Animal physiological hormones studies have vital importance for their health and cortisol is often used to evaluate the stress levels of animals to the environment. In this study, we collected the fecal samples of wild rhesus macaques from Chongzuo. Fecal samples were analyzed for cortisol levels using an enzyme-linked immunosorbent assay. The cortisol levels ranged from 5.02~10.03 μg/g, with a mean value of 7.50 ± 1.77 μg/g. Seasonal variation of fecal cortisol was explored. Results indicated that there were significant seasonal differences (t = 2.969, df = 18, P = 0.008) in the fecal cortisol levels of rhesus macaques. The fecal cortisol level was higher in the rainy season (9.04 ± 0.99) μg/g than in the dry season (6.84 ± 1.55 μg/g). This study evaluated the stress status of rhesus macaques in limestone and provided physiological information, and will help us to get a new perspective for health monitoring.