肾气虚型哮喘大鼠气道炎症指标的研究

Study on Airway Inflammation Index in Asthmatic Rats with Kidney-Qi Deficiency

摘要: 目的：观察肾气虚型哮喘大鼠的一般状态情况、哮喘症状评分与炎症因子IL-6、IL-17、IL-10、IL-35之间的关系，并予全真一气汤干预，探讨评估肾气虚型哮喘大鼠气道炎症的指标。方法：将24只SD雄性大鼠随机分为空白组、模型组、低剂量治疗组(8.33 g/kg)和高剂量治疗组(33.32 g/kg)，每组6只。模型组与低、高剂量治疗组予卵蛋白1 mg、氢氧化铝凝胶10 mg的混悬液1 mL致敏，1%的卵清蛋白超声雾化吸入以激发哮喘，进行惊恐刺激，负重游泳直至力竭等进行肾气虚型哮喘大鼠造模，造模成功后开始药物干预。空白组和模型组予生理盐水3 mL灌服，治疗组按低、高剂量予全真一气汤3 mL灌服，每日1次，连服30天。观察并记录大鼠雾化刺激时的一般状态及行为表现，酶联免疫吸附实验(Enzyme Linked Immunosorbent Assay, ELISA)检测血清IL-6、IL-17、IL-10、IL-35细胞因子含量。结果：与空白组相比，模型组和全真一气汤低、高剂量组大鼠均出现腹式呼吸、呼吸急促、搔鼻、口唇发绀等症状，并伴有毛色暗沉无光泽，蜷缩嗜卧，腹肌痉挛，饮食减少，排便增多等行为状态改变，哮喘症状评分显著升高(P < 0.05)；模型组血清IL-6、IL-17含量明显增加(P < 0.05)，血清IL-10、IL-35的含量明显减少(P < 0.05)；与模型组相比，全真一气汤低、高剂量组的哮喘症状评分显著降低(P < 0.05)，全真一气汤低剂量组、高剂量组血清IL-6、IL-17的含量明显减少(P < 0.05)，IL-35的含量明显增加(P < 0.05)；与全真一气汤低剂量组相比，血清IL-6的含量减少(P < 0.05)，IL-35的含量明显增加(P < 0.05)。结论：肾气虚型哮喘大鼠一般状态情况越差，哮喘症状评分越高，促炎因子IL-6、IL-17的含量越高，抗炎因子IL-35、IL-10的含量越低。

Abstract: Objective: To observe the general state of asthma rats with kidney-qi deficiency, the relationship between asthma symptom score and inflammatory factors IL-6, IL-17, IL-10 and IL-35, and to inter-vene with Quanzhen Yiqi Decoction to explore the indexes for evaluating airway inflammation in asthma rats with kidney-qi deficiency. Methods: Twenty-four SD male rats were randomly divided into blank group, model group, low-dose treatment group (8.33 g/kg) and high-dose treatment group (33.32 g/kg), with 6 rats in each group. The model group and the low-dose and high-dose treatment groups were sensitized with 1 mL of suspension of ovalbumin 1 mg and aluminum hy-droxide gel 10 mg, and 1% ovalbumin was inhaled by ultrasonic atomization to stimulate asthma, and the rats were stimulated by panic, swimming with load until exhaustion, etc., and drug inter-vention was started after the model was successfully established. The blank group and model group were given 3 mL of normal saline, while the treatment group was given 3 mL of Quanzhen Yiqi De-coction at low and high doses, once a day for 30 days. Observe and record the general state and be-havior of rats when they are stimulated by atomization, and detect the contents of IL-6, IL-17, IL-10 and IL-35 cytokines in serum by enzyme linked immunosorbent assay (ELISA). Results: Compared with the blank group, the rats in the model group and the low and high dose groups of Quanzhen Yiqi Decoction all showed symptoms such as abdominal breathing, shortness of breath, scratching nose, cyanosis of mouth and lips, accompanied by behavioral changes such as dull fur, crouching, abdominal muscle spasm, decreased diet and increased defecation, and the asthma symptom score increased significantly (P < 0.05). The contents of serum IL-6 and IL-17 in model group increased significantly (P < 0.05), while the contents of serum IL-10 and IL-35 decreased significantly (P < 0.05). Compared with the model group, the scores of asthma symptoms in low and high doses of Quanzhen Yiqi Decoction were significantly decreased (P < 0.05), and the contents of serum IL-6 and IL-17 in low and high doses of Quanzhen Yiqi Decoction were significantly decreased (P < 0.05), while the content of IL-35 was significantly increased (P < 0.05). Compared with the low-dose group of Quanzhen Yiqi Decoction, the content of serum IL-6 decreased (P < 0.05) and the content of IL-35 increased significantly (P < 0.05). Conclusion: The worse the general state of asthma rats with kid-ney-qi deficiency, the higher the asthma symptom score, the higher the contents of pro-inflammatory factors IL-6 and IL-17, and the lower the contents of anti-inflammatory factors IL-35 and IL-10.