血清双特异性磷酸酶1水平变化在儿童支气管哮喘中的临床意义

Clinical Significance of the Level of Serum Dual-Specificity Phosphatase 1 in Children with Asthma

摘要: 本研究旨在探讨血清DUSP1水平变化在哮喘患儿中的表达及其与哮喘发作风险、严重程度和炎症细胞因子的关系。方法：选取2020年1月至2021年2月在青岛大学附属医院儿童呼吸科就诊的哮喘急性发作期儿童52例、哮喘慢性持续期50例和健康儿童50例进行研究。在青岛大学附属医院检验科，采用酶联免疫吸附法检测血清DUSP1、肿瘤坏死因子-α (TNF-α)、白细胞介素(IL)-1β、IL-6、IL-17水平。结果：① 血清DUSP1水平在健康儿童中最高，在哮喘慢性持续期儿童中次之，在哮喘急性发作期儿童中最低(P < 0.001)。② 血清DUSP1水平变化与哮喘发作风险、急性发作期严重程度呈负相关(P < 0.05)。③ 哮喘急性发作期儿童血清DUSP1水平与TNF-α、IL-1β、IL-17呈负相关(P < 0.05)，与血清IL-6无相关性(P > 0.05)。④ 哮喘慢性持续期儿童血清DUSP1水平与血清TNF-α、IL-1β水平呈负相关(P < 0.05)，与血清IL-6或IL-17无相关性(P > 0.05)。在健康儿童中，血清DUSP1水平与炎症因子无相关性(P > 0.05)。结论：血清DUSP1水平变化可能作为预测哮喘儿童的急性发作的生物学标志物，与哮喘患儿急性发作期、慢性持续期的炎症细胞因子密切相关。

Abstract: The objective of this study was to explore the association between changes in serum DUSP1 expres-sion levels and the risk, severity, and release of relevant inflammatory cytokines in children with asthma. Methods: A total of 152 patients who visited the Department of Pediatric Respirology at The Affiliated Hospital of Qingdao University from January 2020 to February 2021 were selected in this study. Among them, there were 52 children with acute asthma exacerbation, 50 children with chronic persistent asthma, and 50 healthy children. Serum levels of DUSP1, tumor necrosis fac-tor-alpha (TNF-α), interleukin-1 beta (IL-1β), IL-6, and IL-17 were measured using an en-zyme-linked immunosorbent assay in the laboratory of The Affiliated Hospital of Qingdao University. Results: ① The serum DUSP1 level of healthy children was the highest, followed by that of children with chronic persistent asthma, and that of children with acute exacerbation was the lowest (P < 0.001). ② The serum DUSP1 levels were negatively correlated with asthma exacerbation risk and severity during acute exacerbation (P < 0.05). ③ In children with acute asthma exacerbation, se-rum DUSP1 levels were negatively correlated with TNF-α, IL-1β, and IL-17 (P < 0.05), but not corre-lated with serum IL-6 (P > 0.05). ④ In children with chronic persistent asthma, serum DUSP1 levels were negatively correlated with serum TNF-α and IL-1β levels (P < 0.05), but not correlated with serum IL-6 or IL-17 (P > 0.05). In healthy children, serum DUSP1 levels showed no correlation with inflammatory factors (P > 0.05). Conclusion: The serum DUSP1 levels have the potential to serve as a biological marker for predicting acute asthma exacerbation in children and are closely associated with the release of inflammatory cytokines during acute exacerbation and chronic persistent peri-ods in children with asthma.