间充质干细胞在骨折愈合中的研究进展
Research Progress of Mesenchymal Stem Cells in Fracture Healing
DOI: 10.12677/ACM.2023.13102280, PDF,   
作者: 刘 虎:济宁医学院临床医学院,山东 济宁;房好林*:济宁市第一人民医院急诊创伤外科,山东 济宁;济宁市医学科学研究院创伤研究所,山东 济宁
关键词: 间充质干细胞骨折愈合处理措施Mesenchymal Stem Cells Fracture Healing Treatment Measure
摘要: 骨折的临床治疗目的是在最短的时间内获得尽可能好的功能恢复,骨折延迟愈合或不愈合是一个常见的骨折并发症,大约10%的骨折病例中,可能出现骨折延迟愈合或不愈合。这不仅给患者带来巨大的痛苦,还给患者家庭带来一定的经济负担,同时耗费大量的社会医疗资源。如何减轻骨折病人的痛苦,加速骨折的愈合,对患者的快速康复具有重要意义。随着骨折愈合的深入研究,发现间充质干细胞在骨折愈合中发挥至关重要的作用。因此,本文综述了间充质干细胞概念、发展历程,经处理的间充质干细胞在骨折修复中的作用与功能。
Abstract: The purpose of clinical treatment of fracture is to obtain the best possible functional recovery in the shortest time. Delayed or non-union fracture is a common complication of fracture, and delayed or non-union fracture may occur in about 10% of fracture cases. This not only brings great pain to pa-tients, but also brings a certain economic burden to patients’ families, and consumes a lot of social medical resources. How to reduce the pain of fracture patients and accelerate the healing of fracture is of great significance to the rapid recovery of patients. With the further study of fracture healing, it is found that mesenchymal stem cells play a vital role in fracture healing. Therefore, this article re-viewed the concept and development of mesenchymal stem cells, and the role and function of treated mesenchymal stem cells in fracture repair.
文章引用:刘虎, 房好林. 间充质干细胞在骨折愈合中的研究进展[J]. 临床医学进展, 2023, 13(10): 16301-16307. https://doi.org/10.12677/ACM.2023.13102280

参考文献

[1] Karaoz, E., Aksoy, A., Ayhan, S., et al. (2019) Characterization of Mesenchymal Stem Cells from Rat Bone Marrow: Ultrastructural Properties Differentiation Potential and Immunophenotypic Markers. Histochemistry and Cell Biology, 132, 533-546. [Google Scholar] [CrossRef] [PubMed]
[2] 王小璐, 崔宇, 张令强. 促进骨折愈合的治疗策略及机制研究进展[J]. 生命科学, 2021, 33(1): 121-130. [Google Scholar] [CrossRef
[3] 胥少汀, 葛宝丰, 卢世璧. 实用骨科学[M]. 郑州: 河南科学技术出版社, 2019.
[4] 姜壮, 王华松, 丰瑞兵, 等. 生长因子在骨折愈合过程中的作用及其机制的研究进展[J]. 华南国防医学杂志, 2020, 34(11): 823-827. [Google Scholar] [CrossRef
[5] Chen, H., Ghori-Javed, F.Y., Rashid, H., et al. (2014) Runx2 Regulates Endochondral Ossification through Control of Chondro-cyte Proliferation and Differentiation. Journal of Bone and Mineral Research, 29, 2653-2665. [Google Scholar] [CrossRef] [PubMed]
[6] Nishio, Y., Dong, Y., Paris, M., et al. (2016) Runx2-Mediated Regulation of the Zinc Finger Osterix/Sp7 Gene. Gene, 372, 62-70. [Google Scholar] [CrossRef] [PubMed]
[7] Carroll, S.H. and Ravid, K. (2017) Differentiation of Mesenchymal Stem Cells to Osteoblasts and Chondrocytes: A Focus on Adenosine Receptors. Expert Reviews in Molecular Medicine, 15, E1. [Google Scholar] [CrossRef] [PubMed]
[8] 吴钰坤, 韩杰, 温帅波. 骨折愈合过程中Runx2基因的作用机制[J]. 中国组织工程研究, 2021, 25(14): 2274-2279.
[9] Sadatsuki, R., Kaneko, H., Kinoshita, M., Futami, I., Nonaka, R., Culley, K.L., Otero, M., et al. (2017) Perlecan Is Required for the Chondrogenic Differentiation of Synovial Mesenchymal Cells through Regulation of Sox9 Gene Expression. Journal of Orthopaedic Research, 35, 837-846. [Google Scholar] [CrossRef] [PubMed]
[10] Cook, D. and Genever, P. (2013) Regulation of Mesenchymal Stem Cell Differentiation. Advances in Experimental Medicine and Biology, 786, 213-229. [Google Scholar] [CrossRef] [PubMed]
[11] Westendorf, J.J., Kahler, R.A. and Schroeder, T.M. (2014) Wnt Signaling in Osteoblasts and Bone Diseases. Gene, 341, 19-39. [Google Scholar] [CrossRef] [PubMed]
[12] 张鹤令, 景青玲, 宗群川. 人脐带间充质干细胞外泌体对大鼠骨折愈合的影响及其作用机制研究[J]. 中国现代医学杂志, 2022, 32(22): 63-68.
[13] Torrecillas-Baena, B., Pu-lido-Escribano, V., Dorado, G., Gálvez-Moreno, M.Á., Camacho-Cardenosa, M. and Casado-Díaz, A. (2023) Clinical Potential of Mesenchymal Stem Cell-Derived Exosomes in Bone Regeneration. Journal of Clinical Medicine, 12, Article No. 4385. [Google Scholar] [CrossRef] [PubMed]
[14] 娄涵潇, 刘文军, 刘军, 王欣, 张高飞, 王迪, 李佳美. 间充质干细胞预处理策略在严重烧伤治疗中的潜力[J]. 中国组织工程研究, 2023, 27(1): 152-159.
[15] Onder, S., Calikoglu-Koyuncu, A.C., Kazmanli, K., et al. (2018) Magnesium Doping on TiN Coatings Affects Mesenchymal Stem Cell Differentiation and Proliferation Positively in a Dose-Dependent Manner. Bio-Medical Materials and Engineering, 29, 427-438. [Google Scholar] [CrossRef
[16] 张文毓. 生物医用金属材料研究现状与应用进展[J]. 金属世界, 2020(1): 21-27.
[17] Khare, D., Basu, B. and Dubey, A.K. (2020) Electrical Stimulation and Piezoelectric Biomaterials for Bone Tissue Engineering Applications. Biomaterials, 258, Article ID: 120280.
[18] Qi, T., Weng, J., Yu, F., et al. (2021) Insights into the Role of Magnesium Ions in Affecting Osteogenic Differentiation of Mesenchymal Stem Cells. Biological Trace Element Research, 199, 559-567. [Google Scholar] [CrossRef] [PubMed]
[19] Bai, X., Xi, J., Bi, Y., et al. (2017) TNF-α Promotes Survival and Migration of MSCs under Oxidative Stress via NF-κB Pathway to Attenuate Intimal Hyperplasia in Vein Grafts. Journal of Cellular and Molecular Medicine, 21, 2077-2091. [Google Scholar] [CrossRef] [PubMed]
[20] 郝艺. TNF-α诱导的脐带间充质干细胞外泌体对修复细胞及创面愈合的影响研究[D]: [硕士学位论文]. 遵义: 遵义医科大学, 2021.[CrossRef
[21] 王滋润, 梁丽芹, 肖成伟, 郝鹏. Ne-trin-1增强骨髓间充质干细胞治疗骨质疏松性骨折的作用及机制研究[J]. 重庆医科大学学报, 2021, 46(3): 284-288.
[22] Lian, C., Wu, Z., Bo, G., et al. (2016) Melatonin Reversed TNFα-Inhibited Osteogenesis of Human MSCs by Stabilizing SMAD1 Protein. Journal of Pineal Research, 61, 317-327. [Google Scholar] [CrossRef] [PubMed]
[23] Wang, X., Shen, K., Wang, J., et al. (2020) Hypoxic Preconditioning Com-bined with Curcumin Promotes Cell Survival and Mitochondrial Quality of Bone Marrow Mesenchymal Stem Cells, and Accelerates Cutaneous Wound Healing via PGC-1α/SIRT3/HIF-1α Signaling. Free Radical Biology and Medicine, 159, 164-176. [Google Scholar] [CrossRef] [PubMed]
[24] Park, J.S., Suryaprakash, S., Lao, Y.H., et al. (2015) En-gineering Mesenchymal Stem Cells for Regenerative Medicine and Drug Delivery. Methods, 84, 3-16. [Google Scholar] [CrossRef] [PubMed]
[25] Kaddoura, I., Abu-Sittah, G., Ibrahim, A., et al. (2017) Burn In-jury: Review of Pathophysiology and Therapeutic Modalities in Major Burns. The Annals of Fires and Burn Disaster, 30, 95-102.
[26] Liu, L., Song, H., Duan, H., et al. (2016) TSG-6 Secreted by Human Umbilical Cord-MSCs Attenuates Severe Burn-Induced Excessive Inflammation via Inhibiting Activations of P38 and JNK Signaling. Scientific Reports, 6, Article No. 30121. [Google Scholar] [CrossRef] [PubMed]
[27] Redondo-Castro, E., Cunningham, C., Miller, J., et al. (2017) Interleukin-1 Primes Human Mesenchymal Stem Cells towards an Anti-Inflammatory and Pro-Trophic Phenotype in Vitro. Stem Cell Research & Therapy, 8, Article No. 79. [Google Scholar] [CrossRef] [PubMed]
[28] Chen, H., Min, X.H., Wang, Q.Y., et al. (2016) Pre-Activation of Mesenchymal Stem Cells with TNF-α, IL-1β and Nitric Oxide Enhances Its Paracrine Effects on Radiation-Induced In-testinal Injury. Scientific Reports, 5, Article No. 8718. [Google Scholar] [CrossRef] [PubMed]
[29] 陈尔曼. SIRT7调控骨髓间充质干细胞成骨分化和血管生成促进骨修复[D]: [博士学位论文]. 杭州: 浙江大学, 2019.
[30] Zolikha, G., Kashani, I.R., Mohammad, A., et al. (2016) Differentiation of Adipose-Derived Stem Cells into Schwann Cell Phe-notype in Comparison with Bone Marrow Stem Cells. Iranian Journal of Basic Medical Sciences, 13, 76-84.
[31] Pesaresi, M., Sebastian-Perez, R. and Cosma, M.P. (2018) Dedifferentiation, Transdifferentiation and Cell Fusion: In Vivo Reprogramming Strategies for Regenerative Medicine. The FEBS Journal, 286, 1074-1093. [Google Scholar] [CrossRef] [PubMed]
[32] Wang, Z., Huang, M., Zhang, Y., Jiang, X. and Xu, L. (2023) Comparison of Biological Properties and Clinical Application of Mesenchymal Stem Cells from the Mesoderm and Ectoderm. Stem Cells International, 2023, Article ID: 4547875. [Google Scholar] [CrossRef] [PubMed]
[33] Fan, S., Sun, X., Su, C., Xue, Y., Song, X. and Deng, R. (2023) Macrophages-Bone Marrow Mesenchymal Stem Cells Crosstalk in Bone Heal-ing. Frontiers in Cell and Developmental Biology, 11, Article ID: 1193765. [Google Scholar] [CrossRef] [PubMed]