外泌体非编码RNA在非小细胞肺癌中的研究进展
Research Progress of Exosomal Non-Coding RNA in Non-Small Cell Lung Cancer
DOI: 10.12677/HJBM.2023.134045, PDF,    科研立项经费支持
作者: 范 园, 桂海霞, 吴佳琦, 凌振航, 范晓航*：湖北文理学院基础医学院，湖北 襄阳
关键词: 非编码RNA；外泌体；非小细胞肺癌；Non-Coding RNA； Exosome； Non-Small Cell Lung Cancer
摘要: 非小细胞肺癌(non-small cell lung cancer, NSCLC)是最常见的恶性肿瘤之一，死亡率居我国恶性肿瘤首位，患者5年生存率很低。越来越多的研究发现非编码RNA(non-coding RNA, ncRNA)在肿瘤的发生发展中发挥重要作用。外泌体ncRNA可参与转录调节、细胞增殖、凋亡等多个生物学过程。部分外泌体ncRNA在非小细胞肺癌中存在差异表达，有望成为NSCLC早期诊断的标志物。本文主要综述外泌体中的微小RNA(microRNA, miRNA)、长链非编码RNA(long non-coding RNA, lncRNA)和环状RNA(circular RNA, circRNA)在NSCLC中的研究进展，探讨外泌体ncRNA作为早期诊断NSCLC新型标志物的临床应用价值与局限性。
Abstract: Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors, with the highest mortality rate and the very low 5-year survival rate. More and more studies have found that non-coding RNA (ncRNA) plays an important role in the occurrence and development of tumors. Exosomal ncRNA can participate in many biological processes such as transcriptional regulation, cell proliferation, apoptosis and so on. Some exosomal ncRNA are differentially expressed in non- small cell lung cancer, which is expected to be a marker for the early diagnosis of NSCLC. This paper mainly reviews the research progress of microRNAs (miRNA), long non-coding RNA (lncRNA) and circular RNA (circRNA) in exosomes in NSCLC. To investigate the clinical application value and limitation of exosome ncRNA as a novel marker for early diagnosis of NSCLC.
文章引用：范园, 桂海霞, 吴佳琦, 凌振航, 范晓航. 外泌体非编码RNA在非小细胞肺癌中的研究进展[J]. 生物医学, 2023, 13(4): 386-392. https://doi.org/10.12677/HJBM.2023.134045

参考文献

[1] Sung, H., Ferlay, J., Siegel, R.L., et al. (2021) Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 71, 209-249. [Google Scholar] [CrossRef] [PubMed]
[2] 颜晨红, 金儿. 外泌体PD-L1在非小细胞肺癌诊断和治疗上的研究进展[J]. 基础医学与临床, 2023, 43(9): 1457-1461.
[3] Chen, P., Liu, Y., Wen, Y.K. and Zhou, C.C. (2022) Non-Small Cell Lung Cancer in China. Cancer Communications, 42, 937-970. [Google Scholar] [CrossRef] [PubMed]
[4] Anoue, L.T., Tanner, T., Gould, M.K. and Silvestri, G.A. (2015) Lung Cancer Screening. American Journal of Respiratory and Critical Care Medicine, 191, 19-33. [Google Scholar] [CrossRef] 
[5] 程大钊, 唐旭东. 外泌体在肺癌诊疗中的研究进展[J]. 现代肿瘤医学, 2022, 30(4): 731-736.
[6] 陆秋琴, 张琳琳, 贾维. 外泌体作为诊断肺癌的标志物的研究现状[J]. 临床肺科杂志, 2022, 27(2): 276-280.
[7] Nam, B.D., Yoon, S.H., Hong, H., et al. (2021) Tissue Adequacy and Safety of Percutaneous Transthoracic Needle Biopsy for Molecular Analysis in Non-Small Cell Lung Cancer: A Systematic Review and Meta-Analysis. Korean Journal of Radiology, 22, 2082-2093. [Google Scholar] [CrossRef] [PubMed]
[8] Pietersen, P.I., Kristjansdottir, B., Laursen, C., et al. (2022) Systemic Air Embolism following Computed-Tomogra- phy-Guided Transthoracic Needle Biopsy of Lung Lesion—A Systematic Search of Case Reports and Case Series. Acta Radiologica Open, 11. [Google Scholar] [CrossRef] [PubMed]
[9] 陈勇, 杨文魁, 梁志华, 等. CT引导下经皮肺穿刺活检并发症及处理策略的临床研究[J]. 医药论坛杂志, 2019, 40(4): 44-46, 50.
[10] 赵国云, 朱梦迪. 环状RNA在肿瘤免疫中的研究进展[J]. 临床与病理杂志, 2022, 42(3): 726-730.
[11] Kok, V.C. and Yu, C.C. (2020) Cancer-Derived Exosomes: Their Role in Cancer Biology and Biomarker Development. International Journal of Nanomedicine, 15, 8019-8036. [Google Scholar] [CrossRef] 
[12] Kalluri, R. and LeBleu, V.S. (2020) The Biology, Function, and Biomedical Applications of Exosomes. Science, 367, eaau6977. [Google Scholar] [CrossRef] [PubMed]
[13] Chen, J., Li, P., Zhang, T., et al. (2022) Review on Strategies and Technologies for Exosome Isolation and Purification. Frontiers in Bioengineering and Biotechnology, 9, Article 811971. [Google Scholar] [CrossRef] [PubMed]
[14] Xu, W.M., Li, A., Chen, J.J. and Sun, E.J. (2023) Research Development on Exosome Separation Technology. The Journal of Membrane Biology, 256, 25-34. [Google Scholar] [CrossRef] [PubMed]
[15] Wang, J., Yao, Y., Wu, J., et al. (2015) Identification and Analysis of Exosomes Secreted from Macrophages Extracted by Different Methods. International Journal of Clinical and Experimental Pathology, 8, 6135-6142.
[16] Takahashi, K., Chida, S., Suwatthanarak, T., et al. (2022) Non-Competitive Fluorescence Polarization Immunosensing for CD9 Detection Using a Peptide as a Tracer. Lab on a Chip, 22, 2971-2977. [Google Scholar] [CrossRef] 
[17] May, J.M., Bylicky, M., Chopra, S., Coleman, C.N. and Aryankalayil, M.J. (2021) Long and Short Non-Coding RNA and Radiation Response: A Review. Translational Research, 233, 162-179. [Google Scholar] [CrossRef] [PubMed]
[18] Yan, H. and Bu, P. (2021) Non-Coding RNA in Cancer. Essays in Biochemistry, 65, 625-639. [Google Scholar] [CrossRef] 
[19] 陈玲玲, 冯珊珊, 范祖森, 等. 非编码RNA研究进展[J]. 中国科学(生命科学), 2019, 49(12): 1573-1605.
[20] 池诚, 罗毅鑫, 刘洁. 长链非编码RNA在皮肤疾病中的研究进展[J]. 中华医学杂志, 2016, 96(38): 3111-3114.
[21] Li, B., Cao, Y., Sun, M. and Feng, H. (2021) Expression, Regulation, and Function of Exosome-Derived miRNAs in Cancer Progression and Therapy. The FASEB Journal, 35, e21916. [Google Scholar] [CrossRef] 
[22] 何艳玲, 涂红缨. miRNA与非小细胞肺癌诊治相关性的研究进展[J]. 山东医药, 2017, 57(4): 110-112.
[23] 翁克贵, 王颖, 蒋勇. miRNA-21靶向调控Wnt/β-catenin对非小细胞肺癌细胞增殖与侵袭的影响[J]. 中国组织化学与细胞化学杂志, 2018, 27(6): 514-520.
[24] 何鑫, 荣昊, 任伟宏, 等. 外泌体miRNA-21对非小细胞肺癌风险预测价值的研究[J]. 实验与检验医学, 2020, 38(2): 225-230.
[25] Shen, K.H., Hung, J.H., Chang, C.W., et al. (2017) Solasodine Inhibits Invasion of Human Lung Cancer Cell through Downregulation of miR-21 and MMPs Expression. Chemico-Biological Interactions, 268, 129-135. [Google Scholar] [CrossRef] [PubMed]
[26] Li, S., Lin, Y., Wu, Y., et al. (2022) The Value of Serum Exosomal miR-184 in the Diagnosis of NSCLC. Journal of Healthcare Engineering, 2022, Article ID: 9713218. [Google Scholar] [CrossRef] [PubMed]
[27] Yang, F., Yan, Y., Yang, Y., et al. (2020) MiR-210 in Exosomes Derived from CAFs Promotes Non-Small Cell Lung Cancer Migration and Invasion through PTEN/PI3K/AKT Pathway. Cellular Signalling, 73, Article ID: 109675. [Google Scholar] [CrossRef] [PubMed]
[28] Moran, V.A., Perera, R.J. and Khalil, A.M. (2012) Emerging Functional and Mechanistic Paradigms of Mammalian Long Non-Coding RNAs. Nucleic Acids Research, 40, 6391-6400. [Google Scholar] [CrossRef] [PubMed]
[29] Xian, J., Zeng, Y., Chen, S., et al. (2021) Discovery of a Novel linc01125 Isoform in Serum Exosomes as a Promising Biomarker for NSCLC Diagnosis and Survival Assessment. Carcinogenesis, 42, 831-841. [Google Scholar] [CrossRef] [PubMed]
[30] Min, L., Zhu, T., Lv, B., et al. (2022) Exosomal LncRNA RP5-977B1 as a Novel Minimally Invasive Biomarker for Diagnosis and Prognosis in Non-Small Cell Lung Cancer. International Journal of Clinical Oncology, 27, 1013-1024. [Google Scholar] [CrossRef] [PubMed]
[31] Talebi, S., Abadi, A.J., Kazemioula, G., et al. (2022) Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes. Diagnostics, 12, Article 3209. [Google Scholar] [CrossRef] [PubMed]
[32] Hsu, M.T. and Coca-Prados, M. (1979) Electron Microscopic Evidence for Thecircular form of RNA in the Cytoplasm of Eukaryotic Cells. Nature, 280, 339-340. [Google Scholar] [CrossRef] [PubMed]
[33] Xian, J., Su, W., Liu, L., et al. (2020) Identification of Three Circular RNA Cargoes in Serum Exosomes as Diagnostic Biomarkers of Non-Small-Cell Lung Cancer in the Chinese Population. The Journal of Molecular Diagnostics, 22, 1096-1108. [Google Scholar] [CrossRef] [PubMed]
[34] Zhang, N., Nan, A., Chen, L., et al. (2020) Circular RNA circSATB2 Promotes Progression of Non-Small Cell Lung Cancer Cells. Molecular Cancer, 19, Article No. 101. [Google Scholar] [CrossRef] [PubMed]

为你推荐