Rho-Rock信号通路阻断剂对脊髓损伤大鼠神经功能修复的作用

Effect of Rho-Rock Signaling Pathway Block-er on Neurological Recovery in Rats with Spinal Cord Injury

摘要: 目的：探讨Rho-Rock信号通路阻断剂Fasdiual对脊髓损伤(SCI)大鼠神经功能修复的作用。方法：选取8周龄SD (Sprague-Dawley)雄性大鼠36只，采用改良Allen打击法制备脊髓损伤(SCI)大鼠模型后根据实验干预措施随机分组，A组为BMSCs移植组、B组为BMSCs移植 + Fasdiual组、C组为BMSCs移植 + 生长因子组。于SCI术后4周采用BBB (Basso-Beattie-Bresnahan)评分法对SCI大鼠下肢运动功能恢复评分后取脊髓损伤节段制备标本，采用免疫组织化学染色观察对比两组神经干样细胞特性表达蛋白NF200、NSE及胶质细胞GFAP的阳性表达。结果：SCI术后4周各组大鼠下肢运动功能评分均有明显改善，B、C两组SCI大鼠下肢运动功能评分均优于A组，差异有统计学意义(P < 0.05)，B组评分优于A组，两组间比较差异有统计学意义(P < 0.05)；SCI大鼠脊髓标本免疫组化结果显示NF200、NSE在B、C组表达量均高于A组，差异有统计学意义(P < 0.05)，GFAP在A组的表达量高于B、C组，差异均有统计学意义(P < 0.05)。结论：Rho-Rock抑制剂Fasdiual联合骨髓间充质干细胞定向移植后可促进损伤脊髓的修复，促进SCI大鼠神经功能的恢复。

Abstract: Objective: To investigate the effect of the blocker of Rho-Rock signaling pathway (Fasdiual) on neu-rological function in rats with spinal cord injury (SCI). Methods: Thirty-six Sprague-Dawley (SD) male rats aged 8 weeks were selected and prepared by a modified Allen method for spinal cord in-jury (SCI) and randomly divided into groups according to experimental interventions. Group A is BMSCs transplantation group, and group B is BMSCs transplantation with Fasdiual group, C group is BMSCs transplantation with growth factor group. After 4 weeks of SCI, BBB (Basso-Beattie- Bresna-han) scoring method was used to evaluate the recovery of lower extremity motor function of SCI rats. Immunohistochemical staining was used to observe the positive expression of protein NF200, NSE of neural stem cells and GFAP of glial. Results: The motor function scores of lower extremities were significantly improved in all groups after 4 weeks of SCI. The scores of lower extremity motor func-tion of SCI rats in both groups were significantly better than those in group A (P < 0.05). The score of group B was better than that of the group A, there was a statistically significant difference between the two groups (P < 0.05). The immunohistochemistry results of the spinal cord specimens of SCI rats showed that the expression levels of NF200 and NSE in group B and C were higher than those in group A, statistical significance (P < 0.05). The expression of GFAP in group A was higher than that in group B and C. The difference was statistically significant (P < 0.05). Conclusion: Rho-Rock block-er Fasdiual combined with bone marrow mesenchymal stem cells can promote the repair of injured spinal cord and promote the recovery of neurological function in SCI rats.