三叶青黄酮干预荷肺癌小鼠MDSCs生成及相关因子表达的研究

Effect of Trifolium flavone on MDSCs Formation and Expression of Related Factors in Mice with Lung Cancer

摘要: 目的：研究三叶青黄酮对荷肿瘤小鼠MDSCs、PGE2和COX-2的影响，以及它们在调控肿瘤免疫和逆转肿瘤免疫逃逸中的作用。方法：40只C57BL/6小鼠随机分为实验组、环磷酰胺组和三叶青黄酮高、中、低剂量组；每组8只小鼠(以下简称高，中，低)。结果：其它各组的肿瘤体积、肿瘤重量均明显低于模型组(P < 0.05)，外周血中COX-2和PGE2的水平以及脾脏中MDSCs的比例均明显低于模型组(P < 0.05)。小剂量组第8，12，14天的肿瘤体积明显大于环磷酰胺组(P < 0.05)，且外周血中COX-2和PGE2的浓度明显高于小剂量组(P < 0.0.05)，差异有统计学意义。中剂量组在第8天时，肿瘤变大，PGE2在外周血中的含量明显高于对照组(P < 0.05)；高剂量组第14天的瘤体明显缩小(P < 0.05)，与对照组比较差异有统计学意义。中低剂量组的肿瘤抑制率低于对照组(P < 0.05)，而MDSCs的比例高于对照组(P < 0.05)；结论：三叶青黄酮对肿瘤细胞有一定的抑制作用，这种抑制作用可能是通过抑制肿瘤细胞的增殖和凋亡而实现的。

Abstract: Objective: To study the effects of Trifolium flavone on MDSCs, PGE2 and COX-2 in tumor-bearing mice, and their role in regulating tumor immunity and reversing tumor immune escape. Methods: 40 C57BL/6 mice were randomly divided into experimental group, cyclophosphamide group and trifoliate flavonoid high-dose, medium-dose and low-dose groups. There were 8 mice in each group (hereinafter referred to as high, medium and low). Results: The tumor volume and tumor weight in other groups were significantly lower than those in model group (P < 0.05), the levels of COX-2 and PGE2 in peripheral blood and the proportion of MDSCs in spleen were significantly lower than those in model group (P < 0.05). The tumor volume in the low-dose group was significantly higher than that in the cyclophosphamide group at day 8, 12 and 14 (P < 0.05), and the concentrations of COX-2 and PGE2 in peripheral blood were significantly higher than those in the low-dose group (P < 0.05), with statistical significance. On the 8th day, the tumor was enlarged and the PGE2 content in pe-ripheral blood of the medium dose group was significantly higher than that of the control group (P < 0.05). The tumor volume of the high-dose group was significantly reduced on the 14th day (P < 0.05), and the difference was statistically significant compared with the control group. The tumor inhibition rate of medium-low dose group was lower than that of control group (P < 0.05), and the proportion of MDSCs was higher than that of control group (P < 0.05). Conclusion: Trifolium flavone has a certain inhibitory effect on tumor cells, which may be achieved by inhibiting the proliferation and apoptosis of tumor cells.