基于网络药理学和动物实验探讨经络舒宁胶囊治疗DPN的作用机制

To Explore the Mechanism of Jingluoshuning Capsule in the Treatment of DPN Based on Network Pharmacology and Animal Experiments

摘要: 目的：基于网络药理学和动物实验探讨经络舒宁胶囊治疗糖尿病周围神经病变(DPN)的相关分子机制。方法：采用中药系统药理学数据库与分析平台查找经络舒宁胶囊组方中各药味所含的活性成分及对应靶点基因，通过Gene Card等数据库收集DPN的相关疾病靶点基因，Venny 2.1软件获取成分–疾病靶点交集基因，STRING数据库进行蛋白互作(PPI)分析获得核心靶点，利用Metascape数据库进行GO和KEGG富集分析。以2型糖尿病大鼠模型观察经络舒宁胶囊对DPN的治疗作用。结果：数据库检索并筛选得到经络舒宁有效成分41种、潜在靶点584个，DPN疾病相关靶点1869，成分–疾病交集靶点191个，核心靶点36个。GO富集分析得到激素水平调节、磷酸化正调节等分子功能条目，KEGG富集分析结果显示潜在靶点在AGE-RAGE糖尿病并发症、2型糖尿病等信号通路上富集。动物实验表明，与模型组相比，经络舒宁能降低糖尿病大鼠的压痛阈值和甩尾温度阈值，延缓糖尿病大鼠坐骨神经传导速度的减慢，减轻坐骨神经的病理变化。结论：经络舒宁胶囊具有多种活性成分，通过多靶点和多通路改善周围神经功能，进而发挥治疗DPN的作用，动物实验也验证了经络舒宁胶囊对DPN大鼠具有一定的治疗效果。

Abstract: Objective: To explore the molecular mechanism of Jingluoshuning capsule in the treatment of diabetic peripheral neuropathy based on network pharmacology and animal experiments. Methods: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform were used to search the active ingredients and their corresponding target genes in the prescriptions of Jinglu-oshuning capsule. The disease target genes related to diabetic peripheral neuropathy (DPN) were collected through Gene Card database, the component-disease target intersection was obtained by Venny 2.1, and the core targets were obtained by protein interaction (PPI) analysis using STRING database. GO and KEGG enrichment analyses were performed using the Metascape database. To ob-serve the therapeutic effect of Jingluoshuning capsule on peripheral neuropathy in type 2 diabetic rats. Results: 41 active ingredients, 584 potential targets, 1869 disease-related targets, 191 com-ponent-disease intersection targets, and 36 core targets of Jingluoshuning were retrieved and screened in the database. GO enrichment analysis obtained molecular function entries such as hormone level regulation and positive regulation of phosphorylation. KEGG enrichment analysis showed that potential targets were enriched in AGE-RAGE diabetic complications, type 2 diabetes and other signaling pathways. Animal experiments have shown that Jingluoshuning can reduce the tenderness threshold and tail swing temperature threshold of diabetic rats, delay the slowing of sciatic nerve conduction velocity and alleviate the pathological changes of sciatic nerve in diabetic rats. Conclusion: Jingluoshuning has various active ingredients, which can improve peripheral nerve function through multiple targets and pathways, thus playing a role in the treatment of DPN. Ani-mal experiments have also verified that Jingluoshuning has a certain therapeutic effect on DPN rats.