高效液相色谱法快速测定白酒中邻苯二甲酸二丁酯

Rapid Determination of Dibutyl Phthalate in White Liquor by High-Performance Liquid Chromatography

摘要: 目的：建立一种利用高效液相色谱法快速测定白酒中邻苯二甲酸二丁酯的检测方法。方法：样品过0.22 μm微孔滤膜后，氮吹仪挥干后，使用甲醇定容，使用高效液相色谱检测。检测波长225 nm，外标法定量。结果：本方法前处理简便易操作，邻苯二甲酸二丁酯在0.5~20 μg/mL浓度范围内线性关系良好，相关系数均大于0.9999，方法的检出限为0.05 μg/mL，加标回收率分别为93.5%~105.9%，相对标准偏差小于10%。结论：该方法操作简便、准确度高、重现性好，适合白酒中邻苯二甲酸二丁酯的快速测定。

Abstract: Objective: To develop a rapid method for determining dibutyl phthalate (DBP) in white liquor using high-performance liquid chromatography (HPLC). Methods: The samples were filtered through the 0.22 μm microporous membrane and dried using a nitrogen blower. Following methanol adjustment, the HPLC technique was employed for analysis at 225 nm. The external standard method was used for quantitative analysis. Results: The pretreatment method is simple and easy to operate. The method exhibited good linearity in the range of 0.5 to 20 μg/mL for DBP analysis, with correlation coefficients above 0.999. The limit of detection was found to be 0.05 μg/mL, and the average recoveries ranged from 93.5% to 105.9%, with a relative standard deviation of less than 10%. Conclusion: The method is accurate, reproducible, simple and easy to operate, which could be applied to the determination of dibutyl phthalate in white liquor.