补骨脂酊中活性成分补骨脂素、异补骨脂素薄层色谱鉴别

Thinlayer Chromatography Identificatin of Active Components Psoralen and Iso-Psoralen in Psoralen Tincture

摘要: 目的：建立补骨脂酊中补骨脂素、异骨脂素鉴别方法。方法：采用薄层色谱法鉴别补骨脂酊中活性成分补骨脂素和异补骨脂素。结果：采用正乙烷:乙酸乙酯(3:1)、上行展开方式、点样量为1~2 μl，分离效果好，斑点清晰，操作简单快捷，重现性好。结论：通过分析影响薄层色谱鉴别因素，优化补骨脂素、异补骨脂素薄层色谱鉴别方法，为完善医疗机构制剂质量标准提供依据。

Abstract: Objective: To establish the identification method of psoralen and isopsoralen in psoralen tincture. Methods: Active components, namely, Psoralen and isopsoralen, were identified by thin-layer chromatography. Results: Using n-hexane:ethyl acetate (3:1) as the mobile phase, ascending de-velopment mode, and spot volume of 1~2 μl, good separation effect was achieved with clear and dis-tinct spots. The method was simple, fast, and exhibited good reproducibility. Conclusion: By analyz-ing the factors affecting thin-layer chromatography identification, the identification method for psoralen and isopsoralen was optimized, providing a basis for improving the quality standards of pharmaceutical preparations in medical institutions.