甲状腺乳头状癌患者血清样本代谢组学分析

Metabolomics Analysis of Serum Samples from Patients with Papillary Thyroid Cancer

摘要: 目的：分析对比甲状腺乳头状癌(PTC)及健康成人血清样本筛选差异代谢物和异常代谢通路，为PTC的早 期诊疗提供新的思路。方法：选取2022年3月至2022年12月就诊于山东省青岛大学附属中心医院经病理 确诊的PTC患者的血清样本30例，并收集健康成人血清样本36例，利用超高效液相色谱四极杆飞行时间 质谱联用技术，采用主成分分析和偏最小二乘判别分析等方法进行数据处理，筛选差异相关的生物标志 物和代谢通路。结果：通过分析筛选获得41种可信差异代谢物，其中22种下调，19种上调，在PTC中， α-溶血磷脂酸、花生四烯酸等呈下降趋势；顺乌头酸、α-亚麻酸等呈上升趋势。筛选出20条代谢通路， 分别为氮代谢、赖氨酸降解通路、花生四烯酸代谢通路等。结论：PTC和健康成人组代谢轮廓存在显著 差异；糖酵解、氨基酸代谢、脂代谢参与了PTC的病理过程。

Abstract: Objective: To analyze and compare the differential metabolites and abnormal metabolic pathways in serum samples of papillary thyroid carcinoma (PTC) and healthy adults, and provide new ideas for the early diagnosis and treatment of PTC. Method: Thirty serum samples of PTC patients diagnosed with pathology at the Affiliated Central Hospital of Qingdao University in Shandong Province from March 2022 to December 2022 were selected, and 36 healthy adult serum samples were collected. The data were processed using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC), principal component analysis (PCA), partial least squares discriminant analysis (PLSA), and differentially correlated biomarkers and metabolic pathways were screened. Result: 41 credible differential metabolites were obtained through analysis and screening, of which 22 were down regulated and 19 were up regulated. In PTC, α-Lysophosphatidic acid and arachidonic acid showed a downward trend; Shunaconitic acid α-Linolenic acid and other substances are showing an upward trend. 20 metabolic pathways were screened, including nitrogen metabolism, lysine degradation pathway, arachidonic acid metabolism pathway, etc. Conclusion: There is a significant difference in metabolic profile between PTC and healthy adult groups; glycolysis, amino acid metabolism, and lipid metabolism are involved in the pathological process of PTC.