杏鲍菇母种培养基配方优化的试验

Experiment on Optimizing the Formula of Mother Culture Medium for Pleurotus eryngii

摘要: 为了筛选杏鲍菇母种培养基的优化配方，采用单因素试验和正交试验方法，通过设置不同碳源添加量、氮源添加量和无机盐添加量，以菌丝生长速度、菌丝浓度、气生菌丝量和边缘整齐度为主要评价指标，筛选出杏鲍菇母种培养基的最佳配方。结果表明，在培养温度为25˚C，葡萄糖20 g/L，琼脂20 g/L，接种菌块直径为6 mm时，单因素试验筛选得到的玉米芯最适添加量为450 g/L，麦麸最适添加量为60 g/L，无机盐的最适添加量为3.8 g/L；正交试验筛选出的杏鲍菇母种培养基最佳配方为玉米芯450 g/L、麦麸50 g/L、硫酸镁4.2 g/L。利用玉米芯等农业生产废弃物进行杏鲍菇母种栽培，不仅能够节约资源能源，减少环境污染，还能够有效降低成本，变废为宝，提高经济效益。

Abstract: In order to select the optimal formula for the mother culture medium of Pleurotus eryngii, single factor experiments and orthogonal experiments were used. By setting different levels of carbon source, nitrogen source, and inorganic salt addition, the optimal culture formula for the mother culture medium of P. eryngii was selected based on the main evaluation indicators of mycelial growth rate, mycelial concentration, aerial mycelial quantity, and edge uniformity. The results showed that when the cultivation temperature was 25˚C, glucose was 20 g/L, agar was 20 g/L, and the diameter of the inoculum block was 6 mm, the optimal addition amount of corn cob, wheat bran, and inorganic salt was 450 g/L, 60 g/L, and 3.8 g/L, respectively, selected from the single factor experiment; The optimal formula for the mother culture medium of P. eryngii selected through orthogonal experiments is corn cob 450 g/L, wheat bran 50 g/L, and magnesium sulfate 4.2 g/L. Utilizing agricultural production waste such as corn cobs for mother cultivation of P. eryngii can not only save resources and energy, reduce environmental pollution, but also effectively reduce costs, turn waste into treasure, and improve economic benefits.