β-罗勒烯对烟草马铃薯Y病毒防控效果的研究
Research on the Prevention and Control Effect of β-Ocimene on Tobacco Potato virus Y
DOI: 10.12677/HJAS.2023.1312150, PDF,   
作者: 曾维爱*, 翟争光, 蔡海林, 周世民, 谢鹏飞, 李 帆：湖南省烟草公司长沙市公司，湖南 长沙；李宏江*, 林 钢, 阮 颖, 刘春林#：湖南农业大学农学院，湖南 长沙
关键词: β-罗勒烯；植物通讯；马铃薯Y病毒；发病率；病情指数；β-Ocimene； Plant Communication； Potato virus Y； Incidence Rate； Disease Index
摘要: β-罗勒烯是一种能诱导植物产生防御反应的植物通讯信号分子。本文为了探究β-罗勒烯对烤烟马铃薯Y病毒病(PVY)的控制效果，在室内条件下，分析了β-罗勒烯对PVY的发病率及病情指数的影响；在大田条件下，同时分析了β-罗勒烯与常规化学农药对PVY的防控效果。试验结果如下：通过室内盆栽试验分析，β-罗勒烯对PVY具有显著的防控效果，相比于对照发病率下降了67.10%，病情指数下降了70.60%，病毒积累量减少了1.7倍。大田试验结果显示，β-罗勒烯对PVY的控制效果显著优于化学农药，对PVY的控制效果达到58.23%~64.12%；进一步对PVY病情上升率进行统计，结果表明，经β-罗勒烯诱导后PVY的病情指数仅上升了10.0%，显著高于化学农药及对照。以上结果均表明，β-罗勒烯在防控PVY方面具有显著功效。本研究为大田防控PVY提供了新的途径。
Abstract: β-ocimene is a plant communication signaling molecule that can induce plant defense responses. In this paper, in order to investigate the control effect of β-ocimene on tobacco Potato virus Y (PVY) disease, under indoor conditions, the influence of β-ocimene on the incidence rate and disease index of PVY was analyzed; under field conditions, the prevention and control effects of β-ocimene and conventional chemical pesticides on PVY were analyzed simultaneously. The test results are as follows: According to the analysis of indoor pot experiment, β-ocimene has a significant prevention and control effect on PVY. Compared with the control, the incidence rate decreased by 67.10%, the disease index decreased by 70.60%, and the virus accumulation decreased by 1.7 times. The results of field experiments showed that the control effect of β-ocimene on PVY was significantly better than that of chemical pesticides, with a control effect of 58.23%~64.12% on PVY; further statistical analysis of the increase rate of PVY showed that the disease index of PVY induced by β-ocimene only in-creased by 10.0%, significantly higher than that of chemical pesticides and controls. The above results all indicate that β-ocimene has significant efficacy in preventing and controlling PVY. This study provides a new approach to the prevention and control of PVY in the field.
文章引用：曾维爱, 李宏江, 翟争光, 蔡海林, 林钢, 周世民, 谢鹏飞, 李帆, 阮颖, 刘春林. β-罗勒烯对烟草马铃薯Y病毒防控效果的研究[J]. 农业科学, 2023, 13(12): 1100-1107. https://doi.org/10.12677/HJAS.2023.1312150

参考文献

[1] Chen, S., Li, F.X., Liu, D., et al. (2017) Dynamic Expression Analysis of Early Response Genes Induced by Potato vi-rus Y in PVY-Resistant Nicotiana tabacum. Plant Cell Reports, 36, 297-311. [Google Scholar] [CrossRef] [PubMed]
[2] 张凤桐, 程林发, 耿超, 等. 一株PVY[NTN-NW]黑龙江马铃薯分离物的检测鉴定[J]. 植物病理学报, 2019, 49(4): 512-519.
[3] 夏范讲, 郭玉双, 贾蒙骜. 贵州烟田马铃薯Y病毒优势株系全序列克隆及系统进化分析[J]. 中国农业大学学报, 2017, 22(7): 512-519.
[4] 谈文, 刘骏. 烟草病毒病发生流行概况及综合治理的建议[J]. 烟草科技, 1987(2): 33-36.
[5] 段丽娟. 烟草病毒病的发生与防治技术[J]. 现代化农业, 2006(2): 15-16.
[6] 蔡海林, 何命军, 谢鹏飞, 等. 烟草马铃薯Y病毒病的生物防治研究进展[J]. 湖南农业科学, 2012(8): 76-79.
[7] 周佳, 李凤霞, 陈帅, 等. 烟草抗马铃薯Y病毒病相关基因NtPsaN的克隆及表达分析[J]. 中国农业科学, 2010, 43(16): 3323-3330.
[8] 饶思莲, 陶永萍, 赵声春, 等. 烟草抗PVY新品系比较试验[J]. 分子植物育种, 2018, 16(10): 3401-3411.
[9] 刘勇, 许美玲, 黄昌军, 等. 抗马铃薯Y病毒病的烟草种质资源抗性分析[J]. 分子植物育种, 2016, 14(5): 1212-1216.
[10] Morel, J.-B. and Dang, J.L. (1997) The Hypersensitive Response and the Induction of Cell Death in Plants. Cell Death and Differentiation, 4, 671-683. [Google Scholar] [CrossRef] [PubMed]
[11] Durrant, W.E. and Dong, X. (2004) Systemic Acquired Resistance. Annual Review of Phytopathology, 42, 185-209. [Google Scholar] [CrossRef] [PubMed]
[12] 肖牧. 罗勒烯诱导植物防御反应的分子机制研究[D]: [博士学位论文]. 长沙: 湖南农业大学, 2019.
[13] Liu, C.L., Ruan, Y., Guan, C.Y., et al. (2004) β-Ocimene Gene Expression Pattern Induced by Ocimene Signaling Molecule. Science Bulletin, 22, 2373-2374.
[14] Ramegowda, V., Mysore, K.S. and Senthi-Kumar, M. (2014) Virus-Induced Gene Silencing Is a Versatile Tool for Unraveling the Functional Relevance of Multiple Abiotic-Stress-Responsive Genes in Crop Plants. Frontiers in Plant Science, 5, Article 323. [Google Scholar] [CrossRef] [PubMed]

为你推荐