无支架3D工程化脂肪的构建

Construction of Scaffold-Free 3D Engineered Adipose

摘要: 脂肪组织工程是创伤、肿瘤切除后软组织缺损潜在的治疗方法，大多数方法都依赖于使用外源性3D支架来再生脂肪组织。近年来，随着生物制造技术的不断发展，无支架3D工程化脂肪的制备已成为一种备受关注的生物制造方法。由于无支架组织结构不需要细胞粘附在外源材料上并且只涉及细胞和细胞衍生基质，因此无支架组织工程比基于支架的方法提供了许多优势：1) 没有引入任何外源性杂质；2) 小分子扩散、细胞之间的信号传递、细胞迁移不受移植后的影响；3) 仅依赖细胞产生基质。本文综述了脂肪组织工程的三种主要无支架方法：自组装技术，生物反应器和磁悬浮技术，详细阐述了近年来的研究进展及优缺点。

Abstract: Adipose tissue engineering is a potential treatment for soft tissue defects after trauma and tumor resection, most of which rely on the use of exogenous 3D scaffolds to regenerate adipose tissue. In recent years, with the continuous development of biomanufacturing technology, the preparation of stent-free 3D engineered fats has become a kind of biomanufacturing method. Since stentless tissue structures do not require cell adherence to foreign materials and involve only cells and cell-derived matrices, stentless tissue engineering offers many advantages over stent-based approaches: 1) No exogenous impurities are introduced; 2) The diffusion of small molecules, signal transmission be-tween cells and cell migration were not affected by transplantation; 3) Matrix production depends only on cells. In this paper, three main stent-free methods for adipose tissue engineering, self-assembly, bioreactor and magnetic levitation, are reviewed. The research progress, advantages and disadvantages in recent years are described in detail.