STAT4基因多态性与慢性乙型肝炎患者细胞因子分泌水平的相关性研究

The Correlation Study between STAT4 Gene Polymorphism and Cytokine Secretion Levels in Patients with Chronic Hepatitis B

摘要: 目的：通过检测慢性乙型肝炎(chronic hepatitis B virus, CHB)患者信号转导与转录激活因子4 (signal transducer and activator of transcription 4, STAT4)基因的rs7574865位点突变情况，探究CHB患者STAT4表达与细胞因子分泌水平的相关性。方法：收集昆明市第三人民医院2020年8月至2022年8月确诊的300例CHB患者和300例健康体检者为研究对象。采用实时荧光定量PCR技术检测两组STAT4 rs7574865位点的基因多态性。采用酶联免疫吸附法检测CHB患者外周血清IL-21、IL-35、IL-37和TGF-β水平。结果：CHB患者组STAT4基因rs7574865位点的GT、GG基因型高于对照组(P < 0.01)，G等位基因频率显著高于对照组(P < 0.01)。GT型CHB患者IL-21及IL-35水平明显高于TT及GG型(P < 0.05)。结论：STAT4基因多态性与CHB的发生存在一定相关性，GT型患者IL-21、IL-35水平明显升高，临床可通过检测CHB患者STAT4基因多态性及IL-21、IL-35水平评估CHB发生的风险性。

Abstract: Objective: To detect the rs7574865 mutation of signal transducer and activator of transcription 4 (STAT4) gene in patients with chronic hepatitis B (CHB), and explore the correlation between STAT4 expression and cytokine secretion level in CHB patients. Method: A total of 300 CHB patients and 300 healthy individuals diagnosed from August 2020 to August 2022 at Kunming Third Peo-ple’s Hospital were collected as research subjects. Real time fluorescence quantitative PCR technol-ogy was used to detect gene polymorphism at the STAT4 rs7574865 locus in two groups. The IL-21, IL-35, IL-37 and TGF-β in peripheral blood of CHB patients were detected by enzyme-linked im-munosorbent assay. Results: The GT and GG genotypes at rs7574865 of STAT4 gene in patients with CHB were higher than those in the control group (P < 0.01), and the frequency of G allele frequency was significantly higher than that in the control group (P < 0.01). The levels of IL-21 and IL-35 in GT type CHB patients were significantly higher than those in TT and GG types (P < 0.05). Conclusion: There is a certain correlation between STAT4 gene polymorphism and the occurrence of CHB. The levels of IL-21 and IL-35 are significantly increased in GT type patients. Clinical evaluation of the risk of CHB can be achieved by detecting STAT4 gene polymorphism and IL-21 and IL-35 levels in CHB patients.