“金野”紫穗槐石蜡切片制作与显微镜观察分析

Paraffin Sectioning and Microscopic Observation Analysis of Amorpha fruticosa “Jinye”

摘要: 为了探究“金野”紫穗槐和紫穗槐细胞横切结构特征，观察并鉴别“金野”紫穗槐与紫穗槐组织结构的差别，掌握石蜡切片的制作方法。对“金野”紫穗槐及紫穗槐的叶片制作石蜡切片，利用体视显微镜进行染色切片观察。本研究发现，“金野”紫穗槐的叶片横切结构完整，上、下表皮细胞由1层细胞构成，细胞排列整齐、紧密，形状及大小清晰可见。栅栏组织由4层圆形细胞构成，紧密条形堆积排列，细胞间隙隐约可见。紫穗槐的栅栏组织由2层长圆柱细胞构成，形状为条形，边界清楚，排列整齐，大小均匀一致；海绵组织细胞排列分散，体积与厚度不如栅栏组织，与栅栏组织区别明显。“金野”紫穗槐的海绵组织由3~4层小圆形细胞构成；紫穗槐海绵组织较为发达，由3~4层椭圆形、排列疏松细胞构成。两个品种的维管束细胞排列方式相同、大小与形状相似，通过对结果的分析可看出“金野”紫穗槐和紫穗槐的细胞真实性及结构上的差异性。

Abstract: In order to explore the cross-sectional structural characteristics of the cells of “Jinye” purple locust and purple locust, observe and distinguish the differences in tissue structure between “Jinye” pur-ple locust and purple locust, and master the method of making paraffin sections. Make paraffin sec-tions of the leaves of “Jinye” purple locust and purple locust, and observe the staining sections using a stereomicroscope. This study found that the transverse structure of the leaves of “Jinye” purple locust is complete, and the upper and lower epidermal cells are composed of one layer of cells. The cells are arranged neatly and tightly, and the shape and size are clearly visible. The palisade tissue is composed of four layers of circular cells, tightly packed in a linear arrangement, with cell gaps faintly visible. The palisade tissue of Amorpha fruticosa is composed of two layers of long cylindrical cells, with a linear shape, clear boundaries, neat arrangement, and uniform size; Sponge tissue cells are arranged dispersedly, and their volume and thickness are not as good as those of palisade tissue, which is significantly different from palisade tissue The sponge tissue of “Jinye” purple locust is composed of 3~4 layers of small circular cells; The sponge tissue of Amorpha fruticosa is relatively developed, consisting of 3~4 layers of elliptical and loosely arranged cells. The arrangement of vas-cular bundle cells in the two varieties is the same, with similar sizes and shapes. Through analysis of the results, it can be seen that there are differences in cell authenticity and structure between “Jin-ye” purple locust and purple locust.