靶向第二代测序技术在病原体检测中的作用
The Role of Targeted Next-Generation Sequencing in Pathogen Detection
DOI: 10.12677/ACM.2024.141101, PDF,   
作者: 罗 宁*, 何泰瑜, 康 娟, 张大志#:重庆医科大学附属第二医院感染科,重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆医科大学病毒性肝炎研究所,重庆
关键词: 靶向第二代测序感染性疾病病原体诊断Targeted Next-Generation Sequencing Infectious Diseases Pathogen Diagnosis
摘要: 病原学的早期准确识别对于感染性疾病的诊治至关重要。随着基因检测技术的发展,第二代测序技术正被逐步应用于临床病原体的检测。作为第二代测序技术的一个分类,靶向第二代测序技术能选择性地扩增或富集感兴趣的基因组区域,再进行大规模测序,快速准确地检出目标病原体。本文对靶向第二代测序技术在感染性病原体检测中的应用现状进行综述,讨论靶向第二代测序技术作为感染性疾病诊断工具的应用前景。
Abstract: The early and accurate identification of the etiology is crucial for the diagnosis and treatment of in-fectious diseases. With the development of genetic detection technology, the next-generation se-quencing technology (NGS) is gradually being applied to the detection of clinical pathogens. Tar-geted next-generation sequencing (tNGS), as a classification of NGS, can selectively amplify or enrich genomic regions of interest, enabling rapid and accurate detection of the target pathogens through large-scale sequencing. This paper provides a comprehensive review of the application of tNGS in detecting infectious pathogens and explores its potential as a diagnostic tool for infectious diseases.
文章引用:罗宁, 何泰瑜, 康娟, 张大志. 靶向第二代测序技术在病原体检测中的作用[J]. 临床医学进展, 2024, 14(1): 727-735. https://doi.org/10.12677/ACM.2024.141101

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