快速测定植物中可溶性糖含量的方法改良

Improved Method for Rapid Determination and Method Validation of Soluble Sugar Content in Plants

摘要: 可溶性糖含量变化是植物响应逆境胁迫环境的重要指标之一，快速准确检测植物中可溶性糖含量的变化为植物抗逆性能研究提供了技术基础。传统分光光度法测定可溶性糖含量基于以前的实验室配置条件开展，存在测定繁琐和耗时长等问题。本研究基于目前实验室配置条件，分别从植物材料收集、破碎、测定等环节对传统测定方法进行改进，在保证了实验严谨性的前提下，有效提高了实验效率。方法验证结果显示，改进后方法的线性回归方程式为y = 1.2411x + 0.2236，线性相关系数为0.9993，检出限为7.1306 μg/mL，测定下限为28.5224 μg/mL，精密度RSD值小于2%，样品加标回收率为97%~103%之间，验证了改进后方法的可行性。方法应用结果显示，拟南芥和水稻幼苗在冷胁迫下可溶性糖含量均显著升高。改进后的分光光度法测定可溶性糖含量的方法快速准确，适合多个样品的同时测定，可作为可溶性糖含量测定的快速分析方法。

Abstract: The change of soluble sugar content is one of the important indicators for evaluating plant re-sistance in respond to stress environments, and the rapid and accurate detection of soluble sugar content in plants provides a technical basis for studying plant resistance to stress. The traditional method based on the previous laboratory condition is cumbersome and time-consuming. Based on the current laboratory condition, improvements have been made from the aspects of plant material collection, crushing, and detection, which effectively improve the experimental efficiency under the premise of ensuring the rigor of the experiment. The validation results showed a linear regression equation of y = 1.2411x + 0.2236, a linear correlation coefficient of 0.9993, a detection limit of 7.1306 μg/mL, a determination limit of 28.5224 μg/mL, a precision RSD value of less than 2%, and a sample recovery rate of 97%~103%, which proved the feasibility of the improved method. The application results showed that the soluble sugar content in the seedlings of Arabidopsis and rice seedlings are both significantly increased under cold stress. The improved spectrophotometric method for determining soluble sugar content is fast and accurate, suitable for simultaneous de-termination of multiple samples, and can be used for rapid determination of soluble sugar content.