乙肝病毒与类风湿性关节炎关键基因的筛选鉴定

Screening and Identification of Key Genesfor HBV and RA

摘要: 目的：探究乙肝病毒(HBV)与类风湿性关节炎(RA)的共同基因特性。方法：在GEO综合数据库获得HBV 和RA基因芯片数据。采用加权基因共表达网络分析(WGCNA)鉴定HBV和RA相关的共表达模块，并利用 GO进行富集信号通路分析。利用验证队列筛选差异基因。通过免疫组化(IHC)和免疫荧光(IF)的方法对 HBV合并RA患者外周血单个核细胞样本中关键基因进行验证。结果：利用WGCNA筛选出HBV患者和RA 患者外周血中44个共享基因。通过GO分析发现，共享基因富集在锌离子结合、I-kappaB激酶/NF-kappaB 激酶、外源性细胞凋亡的正调控等信号通路。差异分析显示，DYNLT1、GRIPAP1、kiAA2013、MLF2 和TRAFD1在HBV和RA组中均上调。IHC和IF结果显示，与对照组相比，MLF2和TRAFD1表达显著上调， 差异有统计学意义(p < 0.05)。结论：MLF2和TRAFD1可能是HBV与RA的关键基因，这为HBV合并RA 的发病机制、新型生物标志物和潜在靶点的研究提供了参考。

Abstract: Objective: This study aims to identify common genetic characteristics between hepatitis B virus (HBV) and rheumatoid arthritis (RA). Methods: HBV and RA gene chip data were obtained from the GEO comprehensive database. Weighted gene co-expression network analysis (WGCNA) was conducted to identify co-expression modules related to HBV and RA, and GO analysis was used for enrichment signaling pathway analysis. Differential gene screening was performed using a validation cohort. The key genes in peripheral blood mononuclear cell samples of patients with HBV and RA were verified using immunohistochemistry (IHC) and immunofluorescence (IF). Results: WGCNA identified 44 shared genes in the peripheral blood of HBV patients and RA patients. GO analysis revealed that these shared genes were enriched in signaling pathways such as zinc ion binding, I-kappaB kinase/NF-kappaB kinase, and positive regulation of exogenous apoptosis. Differential analysis showed that DYNLT1, GRIPAP1, kiAA2013, MLF2, and TRAFD1 were up-regulated in both the HBV and RA groups. IHC and IF results demonstrated a significant up-regulation of MLF2 and TRAFD1 expression compared to the control group (p < 0.05). Conclusion: MLF2 and TRAFD1 may serve as key genes linking HBV and RA, providing valuable insights for studying the pathogenesis, identifying new biomarkers, and potential targets for HBV combined with RA.