非小细胞肺癌患者Th17细胞、血清IL-17、MMP2的表达在临床病理分期及淋巴结转移的诊断价值

The Diagnostic Value of Th17 Cells, Serum IL-17, and MMP2 Expression in Clinical Pathological Staging and Lymph Node Metastasis in Non-Small Cell Lung Cancer Patients

摘要: 目的：探讨Th17细胞、血清白细胞介素-17 (IL-17)、基质金属蛋白酶(MMP2)在非小细胞肺癌(NSCLC)临床分期及淋巴结转移中的临床应用价值。方法：选取2022年11月~2023年10月入住于陕西省人民医院的42例NSCLC患者(NSCLC组)及40例肺良性病变患者(对照组)，采用流式细胞术检测各组外周血Th17细胞水平，采用酶联免疫吸附法(ELISA)测定血清中IL-17和MMP2水平，评估这三个检测指标在NSCLC临床分期和淋巴结转移中的应用价值及三者联合检测的诊断效能。结果：NSCLC组中，与I~II期患者对比，III~IV期患者外周血Th17细胞水平明显增高，血清IL-17和MMP2水平也明显较高，差异均有统计学意义(均p < 0.05)；NSCLC组中，淋巴结转移者Th细胞水平、血清IL-17和MMP-2水平与未转移者对比，明显较高，差异均有统计学意义(均p < 0.05)；Th17，IL-17和MMP2三项联合检测准确度、特异度、灵敏度分别为95.71%，93.84%和97.22%，与单项检测对比，均明显较高，差异均有统计学意义(均p < 0.05)。结论：Th17细胞，IL-17和MMP2在NSCLC与NSCLC临床分期及淋巴结转移中有一定关联，三者联合检测，有助于NSCLC的鉴别诊断。

Abstract: Objective: To explore the clinical application value of Th17 cells, serum interleukin-17 (IL-17), and matrix metalloproteinases (MMP2) in the clinical staging and lymph node metastasis of non-small cell lung cancer (NSCLC). Methods: 42 NSCLC patients (NSCLC group) and 40 patients with benign lung lesions (control group) admitted to Shaanxi Provincial People’s Hospital from November 2022 to October 2023 were se-lected. Flow cytometry was used to detect peripheral blood Th17 cell levels in each group, and en-zyme-linked immunosorbent assay (ELISA) was used to measure IL-17 and MMP2 levels in the se-rum. The application value of these three detection indicators in clinical staging and lymph node metastasis of NSCLC, as well as the diagnostic efficacy of their combined detection were evaluated. Results: In the NSCLC group, compared with patients in stages I~II, patients in stages III~IV showed a significant increase in peripheral blood Th17 cell levels, as well as significantly higher serum IL-17 and MMP2 levels, with statistical significance (all p < 0.05); in the NSCLC group, the levels of Th cells, serum IL-17, and MMP-2 were significantly higher in patients with lymph node metastasis compared to those without metastasis, and the differences were statistically significant (all p < 0.05); the accuracy, specificity, and sensitivity of the combined detection of Th17, IL-17, and MMP2 were 95.71%, 93.84%, and 97.22%, respectively. Compared with single detection, they were significantly higher, and the differences were statistically significant (all p < 0.05). Conclusion: Th17 cells, IL-17, and MMP2 are associated with clinical staging and lymph node metastasis in NSCLC, and their combined detection can help in the differential diagnosis of NSCLC.