新疆南疆番茄潜叶蛾生殖共生菌分子检测

Molecular Detection of Reproductive Symbiotic Bacteria of Tomato Leafminer in Southern Xinjiang

摘要: 番茄潜叶蛾Tuta absoluta作为入侵害虫，危害程度极其严重，有报道该虫具有孤雌生殖现象。为了探讨其生殖现象，我们重点是分子检测可能与生殖异常有关的内共生菌，并确定其是否会发生孤雌生殖。加入特定引物进行聚合酶链式反应(PCR)特异扩增DNA样本，评估番茄潜叶蛾成虫或幼虫体内是否存在对应共生菌。在孤雌生殖试验中，对处女蛾产卵量、卵孵化率、寿命等数据进行记录，并将其结果与已配对雌性进行比较。我们的研究结果表明，虫体中存在一种内共生菌(沃尔巴克氏菌)；在所进行试验的番茄潜叶蛾种群中，并没有发现孤雌生殖现象，处女蛾只产不孕卵。

Abstract: As an invasive pest, Tuta absoluta (Meyrick) spreads very rapidly and is extremely damaging, and it has been reported that the insect has parthenogenesis. In order to explore its reproductive phe-nomenon, we focused on molecular detection of endosymbionts that may be associated with repro-ductive abnormalities and determine whether parthenogenesis will occur. DNA samples were specifically amplified by polymerase chain reaction (PCR) with the addition of specific primers to assess the presence or absence of the corresponding symbiotic bacteria in T. absoluta adults or larvae. In the parthenogenetic experiment, data on egg production, egg hatchability, and longevity of virgin moths were recorded, and the results were compared with those of paired females. Our results indicate the presence of an endosymbiotic bacterium (Wolbachia) in the worm; in the populations of T. absoluta we tested, we did not find solitary reproduction, and virgin moths laid only infertile eggs.