小胶质细胞核心基因参与脑缺血损伤的生信分析

The Microglia Core Genes Are Involved in Bioinformative Analysis of Cerebral Ischemic Injury

摘要: 目的：探讨小胶质细胞参与脑缺血损伤的核心基因。方法：在R语言中，对GEO数据库中的数据集GSE174574中的单细胞测序数据进行读取、质控、聚类、分群及注释，得到缺血性脑卒中组与假手术组小鼠小胶质细胞的差异表达基因(DEG)，并进行秩和检验、GO富集分析。利用“Seurat”包对小胶质细胞的单细胞测序数据进行再聚类及小胶质细胞拟时序分析。最后使用string网站构建相关mark基因的互作网络图，发现该网络的核心基因。结果：根据缺血性脑卒中单细胞图谱，我们发现小胶质细胞在缺血性脑卒中发挥重要作用，在对小胶质细胞进行二次聚类后，我们鉴定出8种细胞类型，分别为Mic 0、Mic 1、Mic 2、Mic 3、Mic 4、Mic 5、Mic 6、Mic 7。由小胶质细胞拟时序分析结果可知，缺血性脑卒中组的小鼠小胶质细胞向Mic 2亚群发育。进一步鉴定Mic 2的mark基因的互作网络图发现，SPP1在该网络中处于核心地位。结论：小胶质细胞的基因SPP1在脑缺血再灌注损伤中发挥重要作用，可能是治疗缺血性脑卒中的重要靶点。

Abstract: Objective: To investigate the core genes of microglia involved in cerebral ischemic injury. Methods: The differentially expressed genes (DEGs) of mouse microglia in the ischemic stroke group and the sham operation group were obtained by reading, quality control, clustering, clustering and annota-tion of the single-cell sequencing data in the dataset GSE174574 in the GEO database, and the rank-sum test and GO enrichment analysis were performed. The “Seurat” package was used to re-cluster and microglia pseudo-temporal analysis of single-cell sequencing data from microglia. Fi-nally, the STR website was used to construct the interaction network diagram of related mark genes, and the core genes of the network were discovered. Results: According to the ischemic stroke single-cell profile, we found that microglia play an important role in ischemic stroke, and after sec-ondary clustering of microglia, we identified 8 cell types, namely Mic 0, Mic 1, Mic 2, Mic 3, Mic 4, Mic 5, Mic 6, and Mic 7. According to the results of microglial pseudo-chronological analysis, the micro-glia of mice in the ischemic stroke group developed towards the Mic 2 subset. Further identification of the interaction network diagram of the mark gene of Mic 2 showed that SPP1 was at the core of the network. Conclusion: The microglia gene SPP1 plays an important role in cerebral ische-mia-reperfusion injury, and may be an important target for the treatment of ischemic stroke.