艾纳香BbMNR1基因克隆及生物信息学分析

Cloning and Bioinformatics Analysis of BbMNR1 Gene from Blumea balsamifera

摘要: 艾纳香为贵州十大苗药之一，具有较高的药用价值，其萜类化合物可预防虫害、影响花的香味及果实风味。(+)−新薄荷醇脱氢酶(MNR)是一种单萜脱氢酶，参与单萜物质(+)−新薄荷醇和(+)−异薄荷醇的合成。本文通过RT-PCR技术从艾纳香中克隆到BbMNR1基因，并进行生物信息学分析。结果表明，BbMNR1基因完整开放阅读框全长为903 bp，共编码300个氨基酸。BbMNR1序列生物信息学显示，该蛋白分子量为32.57 KDa，理论等电点为5.41，属于非跨膜亲水性稳定蛋白，定位于细胞质。BbMNR1蛋白二级结构中以α-螺旋为主，占总结构的47.67%，与三级结构预测相符。系统进化树分析可得，艾纳香BbMNR1与曼陀罗花(Datura stramonium)和辣椒(Capsicum annuum)亲缘关系最近。本研究将为进一步研究BbMNR1调控萜类化合物生物合成的分子机理奠定基础，同时也为改善艾纳香的品质提供理论支撑。

Abstract: As one of the ten major Miao medicines in Guizhou Province, the terpenoids of the Blumea balsamifera have high medicinal value, which can prevent insect pests and fruit flavor. (+)−neomenthol dehydrogenase (MNR) is a monoterpene dehydrogenase involved in the synthesis of the monoterpenes (+)−neomenthol and (+)−isomenthol. In this study, BbMNR1 was cloned by RT-PCR and subjected to bioinformatic analysis. The results showed that the complete open reading frame of the BbMNR1 gene was 903 bp and encodes 300 amino acids. Bioinformatic analysis showed that the protein was a non-transmembrane hydrophilic stable protein with a molecular weight of 32.57 KDa and a theoretical isoelectric point of 5.41. The secondary structure of BbMNR1 protein is dominated by α-helix, accounting for 47.67% of the total structure, which is consistent with the prediction of the tertiary structure. Phylogenetic tree analysis showed that BbMNR10, Datura stramonium and Capsicum annuum was closely related. This study will lay a foundation for further research of the molecular mechanism of BbMNR1 regulating the biosynthesis of terpenoids and also provide theoretical support for improving the quality of Blumea balsamifera.