糖调节受损模型大鼠胰岛β-细胞功能、胰岛素抵抗水平及胰腺组织中AMPK-mTOR信号通路相关蛋白表达水平变化

Changes of Pancreatic β-Cell Function, Insu-lin Resistance and AMPK-mTOR Signaling Pathway-Related Protein Expression in Pan-creatic Tissues of Rats with Impaired Glucose Regulation Model

摘要: 目的：观察糖调节受损大鼠模型胰岛β-细胞功能、胰岛素抵抗水平及胰腺组织中AMPK-mTOR信号通路相关蛋白表达水平变化。方法：选取82只雄性Wistar大鼠，采用高脂饲料饲养及腹腔注射小剂量链脲佐菌素(STZ)建立糖调节受损(IGR)模型组，普通饲料饲养建立对照组，各组选6只检测血清样本中胰岛素、血糖、2小时后血糖含量，计算胰岛素抵抗指数(HOMA-IR)；利用Western-blot方法检测p-AMPK、p-mTOR、ULK1、LC3、Beclin-1。结果：与对照组比较，模型组大鼠血清FBG、FINS、2 h后血糖、HOMA-IR均升高，大鼠胰腺组织p-AMPK、p-mTOR、ULK1、LC3、Beclin-1表达均升高，差异有统计学意义(P < 0.05)。结论：IGR模型大鼠胰岛素抵抗明显、胰岛β细胞功能降低，AMPK-mTOR信号通路相关蛋白表达升高。

Abstract: Objective: To observe the changes of pancreatic islet β-cell function, insulin resistance level and the expression level of AMPK-mTOR signaling pathway related proteins in pancreatic tissues in the im-paired glucose regulation rat model. Methods: 82 male Wistar rats were selected and fed with high-fat chow and intraperitoneal injection of small trick streptozotocin (STZ) to establish an im-paired glucose regulation (IGR) model group, and a control group was established by normal chow. 6 rats from each group were selected to test the serum samples for insulin, glucose, and glucose level after 2 hours, and calculate the insulin resistance index (HOMA-IR); p-AMPK-mTOR signaling pathway related proteins expression level in pancreatic tissues were detected by Western-blot was used to detect p-AMPK, p-mTOR, ULK1, LC3 and Beclin-1. Results: Compared with the control group, serum FBG, FINS, post-2h glucose, and HOMA-IR were elevated in the model group, and the expres-sion of p-AMPK, p-mTOR, ULK1, LC3, and Beclin-1 was elevated in the pancreatic tissues of the rats, and the difference had a statistical significance (P < 0.05). Conclusion: IGR model rats had obvious insulin resistance, reduced pancreatic β-cell function, and elevated expression of proteins related to AMPK-mTOR signaling pathway.