多功能水凝胶联合富血小板血浆对皮肤修复的研究

Study on Multifunctional Hydrogel Combined with Platelet-Rich Plasma for Skin Repair

摘要: 目的：本实验拟制备一种具有优越的机械性、自愈性、粘附性、光热转化性和细胞相容性的智能纳米复合水凝胶，以增强伤口愈合过程。方法：首先通过柠檬酸钠还原法制备金纳米颗粒(AuNPs)溶液。接着，通过离心法获得富血小板血浆(PRP)，将碳化钛(Ti₃C₂)和金纳米颗粒(AuNPs)引入到血小板水凝胶中，最终获得了金纳米/聚丙烯酰胺/碳化钛/富血小板血浆(AuNPs/PAM/Ti₃C₂/PRP)水凝胶。对水凝胶进行表征，证明其成功制备，并进一步研究其促进皮肤创面愈合的作用。结果：AuNPs/PAM/Ti₃C₂/PRP水凝胶呈疏松多孔状、孔径均一。且水凝胶具有良好的光热转化作用。体外实验证明AuNPs/PAM/Ti₃C₂/PRP水凝胶对细胞无明显毒害作用，而且还能促进细胞的增殖和迁移。结论：成功构建了具有拉伸性、自愈性、粘附性、光热转化性和细胞相容性的AuNPs/PAM/Ti₃C₂/PRP智能复合纳米水凝胶，其展现出良好的促细胞增殖和迁移的作用，在治疗皮肤损伤上具有很大的应用前景，有望成为新型伤口敷料。

Abstract: Objective: In this experiment, it is proposed to prepare a smart nanocomposite hydrogel with superior mechanical, self-healing, adhesion, photothermal conversion and cytocompatibility to enhance the wound healing process. Methods: Firstly, gold nanoparticles (AuNPs) solution was prepared by sodium citrate reduction method. Then, platelet-rich plasma (PRP) was obtained by centrifugation, and titanium carbide (Ti₃C₂) and gold nanoparticles (AuNPs) were introduced into the platelet hydrogel, and finally the gold nano/polyacrylamide/titanium carbide/platelet-rich plasma (AuNPs/PAM/Ti₃C₂/PRP) hydrogel was obtained. The hydrogels were characterized to demonstrate their successful preparation and further investigated for their role in promoting skin wound healing. Results: The AuNPs/PAM/Ti₃C₂/PRP hydrogels were loose and porous with uniform pore size. And the hydrogel has a good photothermal conversion effect. In vitro experiments demonstrated that AuNPs/PAM/Ti₃C₂/PRP hydrogels had no obvious toxic effects on cells and also promoted cell proliferation and migration. Conclusion: AuNPs/PAM/Ti₃C₂/PRP smart composite nanohydrogels with stretchability, self-healing, adhesion, photothermal conversion and cytocompatibility were successfully constructed, which exhibited good pro-cell proliferation and migration, and have great application prospects in the treatment of skin injuries, and are expected to become a new type of wound dressing.