怀山药提取物DM-2治疗C-BSA诱导的慢性肾炎的实验研究

Experimental Study on the Treatment of Dioscorea opposita Thunb. Extract DM-2 on C-BSA Induced Chronic Nephritis Rat Model

摘要: 目的：研究怀山药提取物DM-2对阳离子化牛血清白蛋白(C-BSA)诱导的慢性肾炎模型大鼠的治疗作用。方法：SD大鼠70只，雄性，随机分为正常对照组、慢性肾炎模型组、雷公藤多苷阳性对照组(6.5 mg/kg)、醋酸泼尼松阳性对照组(8.0 mg/kg)以及怀山药提取物DM-2低、中、高剂量(1.0, 2.0, 4.0 g/kg)给药组；采用C-BSA制备肾炎模型大鼠，用怀山药提取物DM-2进行药物干预4周，检测DM-2对肾炎模型大鼠24 h尿蛋白量水平的影响；检测血清总蛋白、胆固醇白蛋白及血清肌酐和尿素氮的含量的影响，同时通过HE染色方法观察DM-2对肾炎模型大鼠肾脏组织病例形态的影响。结果：C-BSA诱导肾炎模型大鼠尿蛋白以及血清肌酐和尿素氮显著增加(P < 0.05)，同时血清白蛋白含量显著降低(P < 0.05)。DM-2低、中、高剂量给药组以及雷公藤多苷和醋酸泼尼松阳性对照组大鼠尿蛋白以及血清肌酐和尿素氮显著降低(P < 0.05)；DM-2中、高剂量给药组以及雷公藤多苷和醋酸泼尼松阳性对照组大鼠血清白蛋白含量显著增加(P < 0.05)。HE染色结果显示，DM-2中、高剂量和醋酸泼尼松给药组大鼠肾脏病变病理特征显著减轻。结论：怀山药提取物DM-2对C-BSA诱导的慢性肾炎大鼠具有良好的治疗作用，能够成为治疗慢性肾炎的候选植物药。

Abstract: Objective: To study the therapeutic effect of Dioscorea opposita Thunb. extract DM-2 on cationized bovine serum albumin (C-BSA) induced chronic nephritis rat model. Methods: 70 SD male rats were randomly divided into normal control group, chronic nephritis model group, tripterygium glycoside positive control group (6.5 mg/kg), prednisone acetate positive control group (8.0 mg/kg) and DM-2 low, medium and high dose groups (1.0, 2.0, 4.0 g/kg). C-BSA was used to prepare nephritis model rats, all administration groups received relevant medicine for consuccessive 4 weeks. The effect of DM-2 on 24 h urinary protein level of nephritis model rats was detected. The contents of serum total protein, cholesterol albumin, serum creatinine and urea nitrogen were detected, effects of DM-2 on the morphology of renal tissue in rats with nephritis were observed by HE staining method. Results: Urinary protein, serum creatinine and urea nitrogen level were significantly increased (P < 0.05), while serum albumin contents were significantly decreased (P < 0.05) in rats with C-BSA induced nephritis rat model. Urine protein, serum creatinine and urea nitrogen in DM-2 low, medium and high dose groups and tripterygium glycosides and prednisone acetate positive control group were significantly decreased (P < 0.05); Serum albumin content in DM-2 medium and high dose groups and tripterygium glycosides and prednisone acetate positive control group were significantly increased (P < 0.05). HE staining results showed that the pathological features of renal lesions were significantly reduced in DM-2 medium, high dose and prednisone acetate groups. Conclusion: DM-2 has a good therapeutic effect on C-BSA-induced chronic nephritis in rats, and can be a candidate plant drug for chronic nephritis.