行气消癥方对子宫肌瘤大鼠治疗作用的研究

Study on the Therapeutic Effect of Xingqi Xiaozheng Prescription on Uterine Fibroids in Rats

摘要: 目的：探讨行气消癥方对子宫肌瘤大鼠激素水平的影响及机制研究。方法：取40只SD大鼠，随机分为正常组、模型组、米非司酮组、行气消癥方低剂量和高剂量组。除正常组外采用雌孕激素联用法造模。治疗后计算子宫系数，HE染色检测子宫组织病理学变化；酶联免疫法检测定血清中的雌二醇(E2)、孕激素(P)、促卵泡素(FSH)、促黄体生成素(LH)水平；免疫组化法和Western-blot法检测子宫组织水通道蛋白1、2、3 (AQP1, 2, 3)的分布和表达水平。结果：模型组大鼠血清E2、P、FSH和LH水平显著升高，子宫系数高于正常组。与模型组相比，各给药组子宫系数降低，病理变化有不同程度改善，子宫AQP1、2、3表达水平显著降低(P < 0.05)。行气消癥方高剂量组大鼠血清E2、P、FSH和LH水平显著下调(P < 0.01)。结论：行气消癥方可下调血清性激素水平改善子宫肌瘤，其机制可能与下调子宫AQP1、2、3的表达相关。

Abstract: Objective: To investigate the effect of Xingqi Xiaozheng prescription on hormone levels in rats with uterine fibroids and its mechanism. Methods: 40 SD rats were randomly divided into normal group, model group, Mifepristone group, low dose and high dose Xingqi Xiaozheng prescription groups. In addition to the normal group, the model was established by the combination of estrogen and progesterone. After treatment, uterine coefficient was calculated, and the histopathological changes of uterus were detected by HE staining. The levels of estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH) and luteinizing hormonein (LH) serum were detected by enzyme-linked immunosorbent assay. The distribution and expression levels of aquaporin 1, 2 and 3 (AQP1, 2 and 3) in uterine tissue were detected by immunohistochemistry and Western-blot. Results: The levels of serum E2, P, FSH and LH in model group were significantly increased, and the uterine coefficient was higher than that in normal group. Compared with model group, the uterine coefficient was significantly decreased, the pathological changes were improved in different degrees, and the expression levels of AQP1, 2 and 3 in uterus were significantly decreased (P < 0.05). The serum levels of E2, P, FSH and LH in Xingqi Xiaozheng prescription group were significantly decreased (P < 0.01). Conclusion: Xingqi Xiaozheng prescription can improve uterine fibroids by down-regulating serum sex hormone levels, and its mechanism may be related to down-regulating the expression of uterine AQP1, 2 and 3.