胶体果胶铋胶囊中铋在小鼠部分器官中分布初探

A Preliminary Study on the Distribution of Bismuth in Colloidal Pectin Bismuth Capsules in Some Organs of Mice

摘要: 目的：建立测量胶体果胶铋胶囊经灌胃后24 h铋在小鼠心、肝、脾、肺、肾、小肠、脑、粪中的含量测定方法。方法：采用湿消化法–火焰原子吸收法测定小鼠灌胃胶体果胶铋胶囊24 h后心、肝、脾、肺、肾、小肠、脑、粪中的铋含量，与给药前进行比较。结果：湿法消解后各器官样品体积浓度范围在0.09701~33.4851 mg/L，浓度范围内与吸光度值呈良好线性关系(n = 8)，建立的标准工作曲线相关系数r = 0.9999，精密度试验RSD = 1.7971%，重复性试验RSD = 1.9723%，加样回收率平均值为101.1002% (RSD = 2.0568%, n = 9)，小鼠灌胃胶体果胶铋胶囊24 h后有肾、小肠、粪中的铋含量有显著性差异。结论：该测量方法简单、准确、重复性好，回收率高，可用于胶体果胶铋胶囊经口服用后各器官中铋含量的测定；胶体果胶铋经灌胃给小鼠后，24小时，铋在小鼠部分脏器和材料中的分布是粪便中最多，依次是肠、肾、心、脾、肝、肺、脑组织最少。

Abstract: Objective: To establish a method for the determination of the bismuth contents of the heart, liver, spleen, lungs, kidneys, small intestine, brain, and stool of mice 24 hours after intragastric administration of colloidal bismuth pectin capsules. Methods: The contents of bismuth in the heart, liver, spleen, lungs, kidneys, small intestine, brain, and stool of mice after 24 hours of intragastric administration of colloidal bismuth pectin capsules were determined by the wet digestion method and the flame atomic absorption method. Results: After wet digestion, the volume concentration of each organ sample ranged from 0.09701~33.4851 mg/L, and there was a good linear relationship between the concentration range and the absorbance value (n = 8). The correlation coefficient of the established standard working curve was r = 0.9999, the precision test result was RSD = 1.79971%, and the repeatability test result was RSD = 1.9723%. The average recovery rate was 101.1002% (RSD = 2.0568%, n = 9). There were significant differences in the contents of bismuth in the kidneys, small intestine, and stool after 24 hours of intragastric administration of bismuth gel pectin capsules. Conclusion: The method described here is simple, accurate, reproducible, and has a high recovery rate. It can be used for the determination of the bismuth contents of various organs after oral administration of colloidal bismuth pectin capsules. After intragastric administration of colloidal bismuth pectin to mice, the highest concentration of bismuth was detected in the feces, followed by the intestine, kidneys, heart, spleen, liver, lungs, and brain.