脐带血间充质干细胞对角膜新生血管形成的抑制作用

Inhibitory Effect of Umbilical Cord Mesenchymal Stem Cells on Corneal Neovascularization

摘要: 目的：研究球结膜下注射人脐带血来源的间充质干细胞悬液治疗角膜碱烧伤新生血管的有效性。方法：本研究将30只新西兰家兔制备右眼碱烧伤模型，随机分实验组和对照组各15只(15眼)。实验组球结膜下注射脐带血来源间充质干细胞悬液，对照组不给予球结膜下治疗。采用裂隙灯生物显微镜观察并拍照记录注射后第5天，14天角膜新生血管情况，计算角膜新生血管面积。取两组角膜进行HE染色，观察角膜上皮愈合情况。结果：实验结果显示，第5天实验组和对照组之间的角膜新生血管面积差异没有显著性统计意义(P > 0.05)，但第14天实验组的新生血管面积显著小于对照组(P < 0.05)。结论：本研究结果证明，球结膜下注射人脐带血间充质干细胞可抑制角膜新生血管并促进碱烧伤角膜上皮的修复。

Abstract: Objective: To study the effectiveness of subconjunctival injection of mesenchymal stem cell suspension derived from human umbilical cord blood in the treatment of neovascularization in corneal alkali burns. Methods: In this study, 30 rabbits in New Zealand were prepared right eye alkaline burn models and randomly divided into experimental and control groups (15 eyes). Subconjunctival injection of umbilical cord blood derived mesenchymal stem cell suspension in the experimental group was not followed by subconjunctival treatment in the control group. New corneal blood vessels were observed and photographed using a fissure biopsy 5 and 14 days after injection. Two groups of corneas were taken for HE staining to observe the healing of corneal epithelium. Results: There was no statistically significant difference in the area of new corneal vessels between day 5 and control group (P > 0.05), but the area of new corneal vessels was significantly smaller in day 14 group than in control group (P < 0.05). Conclusion: Our results demonstrate that subconjunctival injection of human umbilical cord mesenchymal stem cells inhibits new corneal vessels and promotes repair of alkali burned corneas.