核仁素在结肠癌组织中的表达及临床意义

Expression and Clinical Significance of Nucleolus in Colon Cancer Tissue

摘要: 目的：核仁素(Nucleolin, NCL)已被证明在肿瘤中发挥重要作用，但结肠癌中NCL表达情况尚不明确。本研究旨在探究NCL在结肠癌中的表达情况与结肠癌临床病理特征以及预后的关系。方法：回顾性分析了青岛大学附属医院胃肠外科的184例结肠癌患者病历资料。采用免疫组织化学法检测研究对象组织标本的NCL表达情况。使用SPSS软件进行统计学分析。采用卡方检验比较NCL表达与临床特征间的关系。绘制生存曲线比较组间生存差异。使用Cox比例风险模型进行单因素和多因素分析。结果：细胞核NCL的高低表达与患者的脉管侵犯、T分期、N分期以及TNM分期有关；细胞膜NCL的高低表达与肿瘤大小、大体类型、脉管侵犯、T分期、N分期、M分期和TNM分期有关。与NCL高表达患者相比，NCL低表达患者预后总生存期更好。单因素Cox分析结果显示，肿瘤部位、肿瘤大小、脉管侵犯、TNM分期、细胞核NCL表达情况、细胞膜NCL表达情况为结肠癌患者总生存期的影响因素。多因素结果发现：肿瘤部位、TNM分期、NCL表达情况是影响结肠癌患者生存的独立预后因素。结论：结肠癌组织中NCL的高表达会导致较差的预后。膜NCL高表达与肿瘤的侵袭性相关。NCL与结肠癌患者的OS密切相关，是影响结肠癌患者预后的独立危险因素。

Abstract: Objective: Nucleolin (NCL) has been shown to play an important role in tumors, but the expression of NCL in colon cancer is still unclear. The aim of this study is to explore the relationship between the expression of NCL in colon cancer and the clinical pathological characteristics and prognosis of colon cancer. Method: A retrospective analysis was conducted on the medical records of 184 patients with colon cancer from the Department of Gastrointestinal, Affiliated Hospital of Qingdao University. Immunohistochemical method was used to detect the expression of NCL in the tissue samples of the study subjects. Perform statistical analysis using SPSS software. Use chi square test to compare the relationship between NCL expression and clinical features. Draw survival curves to compare survival differences between groups. Use Cox proportional risk model for single factor and multi factor analysis. Result: The high and low expression of nuclear NCL is related to the patient’s vascular invasion, T stage, N stage, and TNM stage; the high and low expression of cell membrane NCL is related to tumor size, gross type, vascular invasion, T stage, N stage, M stage, and TNM stage. Compared with patients with high expression of NCL, patients with low expression of NCL have a better overall survival prognosis. The results of univariate Cox analysis showed that tumor location, tumor size, vascular invasion, TNM staging, nuclear NCL expression, and cell membrane NCL expression were the influencing factors on the overall survival of colon cancer patients. Multivariate results showed that tumor location, TNM staging, and NCL expression were independent prognostic factors affecting the survival of colon cancer patients. Conclusion: High expression of NCL in colon cancer tissue can lead to poor prognosis. High expression of membrane NCL is associated with tumor invasiveness. NCL is closely related to the OS of colon cancer patients and is an independent risk factor affecting the prognosis of colon cancer patients.