OsWRKY45影响水稻抗旱性
OsWRKY45 Affects the Drought Resistance of Rice
DOI: 10.12677/hjas.2024.144060, PDF,   
作者: 孔 丽:浙江师范大学生命科学学院,浙江 金华
关键词: OsWRKY45耐旱性SPADOsWRKY45 Drought Tolerance SPAD
摘要: 本文以野生型水稻石狩白毛(SSBM)和OsWRKY45过表达(35S-WRKY45-Flag)为实验材料,使用PEG6000模拟干旱处理,研究其在干旱条件下表达模式及生理指标。结果表明,PEG处理诱导了WRKY45的表达,35S-WRKY45-Flag的光合速率、气孔导度及蒸腾速率与野生型相比显著降低,且35S-WRKY45-Flag增加了水稻的耐旱性;PEG处理两天后,SSBM的SPAD值、地上部鲜重与根鲜重都显著降低,而35S-WRKY45-Flag处理后SPAD值与根鲜重无显著变化,地上部鲜重显著降低,且处理后35S-WRKY45-Flag的SPAD值、地上部鲜重与根鲜重都显著高于处理后的SSBM。总之,35S-WRKY45-Flag在一定程度上增加了水稻耐旱性。
Abstract: This article uses wild-type rice variety SSBM and OsWRKY45 over expression (35S-WRKY45-Flag) as experimental materials, and uses PEG6000 to simulate drought treatment to study their expression patterns and physiological indicators under drought conditions. The results showed that PEG treatment induced the expression of WRKY45, and the photosynthetic rate, stomatal conductance, and transpiration rate of 35S-WRKY45-Flag were significantly reduced compared to the wild type. Moreover, 35S-WRKY45-Flag increased the drought resistance of rice; after two days of PEG treatment, the SPAD value, fresh aboveground weight, and fresh root weight of SSBM significantly decreased, while there was no significant change in SPAD value and fresh root weight after treatment with 35S-WRKY45-Flags. The fresh aboveground weight significantly decreased, and the SPAD value, fresh aboveground weight, and fresh root weight of 35S-WRKY45-Flags were significantly higher than those of SSBM after treatment. In summary, 35S-WRKY45-Flag has to some extent increased the drought tolerance of rice.
文章引用:孔丽. OsWRKY45影响水稻抗旱性[J]. 农业科学, 2024, 14(4): 472-480. https://doi.org/10.12677/hjas.2024.144060

参考文献

[1] FAO (2015) Statistical Pocketbook. Food and Agriculture Organization of the United Nations.
[2] Donde, R., et al. (2019) Computational Characterization of Structural and Functional Roles of DREB1A, DREB1B and DREB1C in Enhancing Cold Tolerance in Rice Plant. Amino Acids, 51, 839-853. [Google Scholar] [CrossRef] [PubMed]
[3] Rijsberman, F.R. (2006) Water Scarcity: Fact or Fiction? Agricultural Water Management, 80, 5-22. [Google Scholar] [CrossRef
[4] Hao, Z., Singh, V.P. and Xia, Y. (2018) Seasonal Drought Prediction: Advances, Challenges, and Future Prospects. Reviews of Geophysics, 56, 108-141. [Google Scholar] [CrossRef
[5] Oladosu, Y., et al. (2019) Drought Resistance in Rice from Conventional to Molecular Breeding: A Review. International Journal of Molecular Sciences, 20, 3519. [Google Scholar] [CrossRef] [PubMed]
[6] Blum, A. (2011) Plant Breeding for Water-Limited Environments. Plant Breed. Water Limited Environments, 1-255. [Google Scholar] [CrossRef
[7] Todaka, D., et al. (2017) Temporal and Spatial Changes in Gene Expression, Metabolite Accumulation and Phytohormone Content in Rice Seedlings Grown under Drought Stress Conditions. The Plant Journal, 90, 61-78. [Google Scholar] [CrossRef] [PubMed]
[8] Hassan, M.A., et al. (2023) Drought Stress in Rice: Morpho-Physiological and Molecular Responses and Marker-Assisted Breeding. Frontiers in Plant Science, 14, Article ID: 1215371. [Google Scholar] [CrossRef] [PubMed]
[9] Kummu, M. et al. (2016) The World’s Road to Water Scarcity: Shortage and Stress in the 20th Century and Pathways towards Sustainability. Scientific Reports, 6, Article Number: 38495. [Google Scholar] [CrossRef] [PubMed]
[10] Santini, M., Noce, S., Antonelli, M. and Caporaso, L. (2022) Complex Drought Patterns Robustly Explain Global Yield Loss for Major Crops. Scientific Reports, 12, Article Number: 5792. [Google Scholar] [CrossRef] [PubMed]
[11] Inoue, H., et al. (2013) Blast Resistance of CC-NB-LRR Protein Pb1 Is Mediated by WRKY45 through Protein-Protein Interaction. Proceedings of the National Academy of Sciences, 110, 9577-9582. [Google Scholar] [CrossRef] [PubMed]
[12] Shimono, M., et al. (2007) Rice WRKY45 Plays a Crucial Role in Benzothiadiazole-Inducible Blast Resistance. The Plant Cell, 19, 2064-2076. [Google Scholar] [CrossRef] [PubMed]
[13] Tao, Z., et al. (2009) A Pair of Allelic WRKY Genes Play Opposite Roles in Rice-Bacteria Interactions. Plant Physiology, 151, 936-948. [Google Scholar] [CrossRef] [PubMed]
[14] Xu, L., et al. (2023) AIM1-Dependent High Basal Salicylic Acid Accumulation Modulates Stomatal Aperture in Rice. New Phytologist, 238, 1420-1430. [Google Scholar] [CrossRef] [PubMed]
[15] Food and Agricultural Organization (2020) Food Outlook—Biannual Report on Global Food Markets. FAO, Rome.
[16] Nahar, S., Kalita, J., Sahoo, L. and Tanti, B. (2016) Morphophysiological and Molecular Effects of Drought Stress in Rice. Annals of Plant Sciences, 5, No. 9. [Google Scholar] [CrossRef
[17] Kadam, N.N., et al. (2017) Genetic Control of Plasticity in Root Morphology and Anatomy of Rice in Response to Water Deficit. Plant Physiology, 174, 2302-2315. [Google Scholar] [CrossRef] [PubMed]
[18] Quinones, C., Mattes, N., Faronilo, J., Sudhir, Y. and Jagadish, K.S.V. (2017) Drought Stress Reduces Grain Yield by Altering Floral Meristem Development and Sink Size under Dry-Seeded Rice Cultivation. Crop Science, 57, 2098-2108. [Google Scholar] [CrossRef