两株萘降解菌的降解特性及动力学分析

Degradation Characteristics and Kinetic Analysis of Two Strains of Naphthalene-Degrading Bacteria

摘要: 研究不同萘初始浓度条件下，铜绿假单胞菌ZS1和红球菌b1对萘的降解特性。ZS1和b1菌株最适萘初始质量浓度为分别为200 mg/L和300 mg/L。培养120 h菌株ZS1和b1对萘的降解率分别达到80.45%和87.13%。通过动力学拟合，证实两种菌株对萘的降解过程均符合一级降解动力学方程，降解常数K分别为0.01843 kd/h⁻¹和0.02832 kd/h⁻¹。通过气相色谱–质谱(GC-MS)分析了萘降解阶段的中间产物，并推测了两种菌株降解萘的途径分别为邻苯二甲酸途径和水杨酸途径。

Abstract: The degradation characteristics of naphthalene by Pseudomonas aeruginosa ZS1 and Rhodococcus b1 under different initial concentrations of naphthalene were studied. The optimum initial naphthalene concentrations of ZS1 and b1 were 200 mg/L and 300 mg/L, respectively. After 120 hours of culture, the degradation rate of naphthalene by strain ZS1 and b1 reached 80.45% and 87.13%, respectively. Through kinetic fitting, it was confirmed that the degradation process of naphthalene by the two strains conformed to the first-order degradation kinetic equation, and the degradation constants K were 0.01843 kd/h⁻¹ and 0.02832 kd/h⁻¹, respectively. The intermediate products of naphthalene degradation stage were analyzed by gas chromatography-mass spectrometry (GC-MS), and the pathways of naphthalene degradation by the two strains were speculated to be phthalic acid pathway and salicylic acid pathway.