血小板细胞膜仿生纳米系统在烟曲霉菌性角膜炎中的抗炎作用
Anti-Inflammatory Effect of Platelet Membrane Biomimetic Nanosystem in Aspergillus fumigatus Keratitis
摘要: 目的：构建一种血小板细胞膜包覆聚乳酸–羟基乙酸聚合物(poly (lactic-co-glycolic acid), PLGA)的仿生纳米系统(PLTm@PLGA)，评估其在烟曲霉菌性角膜炎中的抗炎作用。方法：制备并表征PLTm@PLGA。通过CCK-8、溶血试验检测生物安全性。实时荧光定量聚合酶链反应(RT-qPCR)检测烟曲霉菌刺激RAW264.7细胞后炎症因子TNF-α、IL-1β的mRNA表达水平。建立烟曲霉菌性角膜炎小鼠模型，观察治疗效果并进行炎症评分。结果：PLTm@PLGA呈球形，表现为典型的“壳–核”结构，其粒径为234.3 ± 8.9 nm。PLGA、PLTm@PLGA无明显细胞毒性，血液相容性好。PLTm@PLGA显著抑制RAW264.7细胞中TNF-α、IL-1β mRNA表达。PLTm@PLGA处理组临床炎症评分显著下降。结论：PLTm@PLGA具有良好生物相容性，抑制促炎细胞因子表达，减轻了小鼠烟曲霉菌性角膜炎的炎症反应。
Abstract: Objective: Poly (lactic acid-co-glycolic acid) (PLGA) was coated with platelet membranes to construct a biomimetic NanoSystem (PLTm@PLGA), which was evaluated its protective effect in Aspergillus fumigatus keratitis. Methods: PLTm@PLGA was prepared and characterized. The biosafety was investigated by CCK-8 and hemolysis tests. The mRNA expression levels of TNF-α and IL-1β were detected by Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) in RAW264.7 cells stimulating with Aspergillus fumigatus. Mice models of Aspergillus fumigatus keratitis were established to observe the therapeutic effect and score the inflammation. Results: PLTm@PLGA is spherical and has a typical “shell-core” structure, and its particle size was 234.3 ± 8.9 nm. PLGA and PLTm@PLGA showed no significant cytotoxicity and hemolysis. PLTm@PLGA significantly inhibited the mRNA expression of TNF-α and IL-1β in RAW264.7 cells. The clinical inflammation score was significantly decreased in PLTm@PLGA group. Conclusion: PLTm@PLGA has great biosafety, and plays an anti-inflammatory role in Aspergillus fumigatus keratitis in mice by inhibiting the expression of inflammatory cytokines.
文章引用：张芸凤, 刘星, 田怡然, 林静. 血小板细胞膜仿生纳米系统在烟曲霉菌性角膜炎中的抗炎作用[J]. 临床医学进展, 2024, 14(4): 2045-2054. https://doi.org/10.12677/acm.2024.1441261

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