鼠妇提取物及不同极性部位缓解神经病理性疼痛作用研究

Analgesic Activities of Different Polarity Fractions of the Aqueous Extract from Armadillidium vulgare Latreille

摘要: 目的：比较鼠妇水提物及不同极性部位缓解神经病理性疼痛的药效作用，为鼠妇临床应用和镇痛药效物质的深入研究奠定基础。方法：鼠妇经水提得到水提物(AVW)后，依次用石油醚、乙酸乙酯萃取得到石油醚部位(APF)、乙酸乙酯部位(AEF)和水部位(AWF)。此外将AVW经石油醚脱脂后通过硅胶柱层析分段，制备获得石油醚–乙酸乙酯(3:1)、石油醚–乙酸乙酯(1:1)、石油醚–乙酸乙酯(1:3)、乙酸乙酯、甲醇洗脱部位。建立大鼠坐骨神经慢性压迫损伤模型，灌胃(0.5 g/kg)或鞘内(0.2 mg/20μL)给予鼠妇提取物或不同部位样品，运用Von Frey及Hargreaves分别测定大鼠机械及热痛阈值，评价鼠妇水提物及不同极性部位的镇痛活性。结果：鼠妇水提物有效缓解神经病理性疼痛，且不同极性部位镇痛活性有差异。结论：石油醚–乙酸乙酯(1:1)洗脱部位和甲醇洗脱部位具有较强的镇痛活性，为主要镇痛活性部位。

Abstract: Objective: The aim was to compare the analgesic effect of the aqueous extract and its different polarity fractions of Armadillidium vulgare Latreille on neuropathic pain, which laid a foundation for further study on clinical application and analgesic substances of Armadillidium vulgare Latreille. Methods: After AVW was obtained, APF, AEF and AWF were extracted with petroleum ether and ethyl acetate successively. Then, AVW defatted by petroleum ether was segmented by silica gel column chromatography to obtain petroleum ether-ethyl acetate (3:1), petroleum ether-ethyl acetate (1:1), petroleum ether-ethyl acetate (1:3), ethyl acetate, methanol elution parts. The model of chronic constriction injury of the sciatic nerve in rats was established. After oral administration (0.5 g/kg) or intrathecal administration (0.2 mg/20μL), the mechanical and thermal pain thresholds of rats were determined by Von Frey and Hargreaves respectively to evaluate the analgesic activities of AVW and its different polarity fractions. Results: AVW effectively relieved neuropathic pain, and the analgesic activity of different polarity fractions is different. Conclusion: Petroleum ether-Ethyl acetate (1:1) eluting site and methanol eluting site had strong analgesic effect, and were the main analgesic active fractions.