MCM4在结直肠癌中的表达及意义

Expression and Significance of MCM4 in Colorectal Cancer

摘要: 目的：分析MCM4在结直肠癌细胞中的表达情况，研究MCM4对结直肠癌细胞的作用，进一步探讨MCM4和结直肠癌(CRC)之间的联系。方法：1) 采用免疫组织化学法比较MCM4在不同结肠组织中的表达情况，分析MCM4与结直肠癌患者临床病理之间的关系。2) 生存分析使用Kaplan-Meier方法，logrank检验评估生存差异。采用Cox回归分析确定MCM4对CRC的预测价值。3) 采用qRT-PCR和Western Blot检测MCM4在结直肠癌细胞系HCT 116和Caco2中的表达水平。4) 构建敲低MCM4的表达载体，建立敲低MCM4的表达细胞系。通过CCK-8细胞增殖实验、细胞克隆形成实验检测CRC细胞的增殖能力。结果：1) MCM4在CRC组织和细胞中的表达水平高于正常结直肠组织和细胞。2) MCM4可促进结直肠癌细胞的增殖能力。3) MCM4的高表达与患者的预后不良显著相关。结论：MCM4促进结直肠癌的发生发展，可能是有效的结直肠肿瘤标志物和潜在的治疗靶点。

Abstract: Objective: To analyze the expression of MCM4 in colorectal cancer cells, study the role of MCM4 on colorectal cancer cells, and further explore the association between MCM4 and colorectal cancer (CRC). Methods: 1) Immunohistochemistry was used to compare the expression of MCM4 in different colon tissues and analyze the relationship between MCM4 and clinicopathology in colorectal cancer patients. 2) Survival analysis was performed using the Kaplan-Meier method with logrank test to assess survival differences. Cox regression analysis was used to determine the predictive value of MCM4 for CRC. 3) The expression levels of MCM4 in colorectal cancer cell lines HCT 116 and Caco2 were detected by qRT-PCR and Western Blot. 4) To construct an expression vector that knocks down MCM4 and establish a cell line expressing the knocked-down MCM4. The proliferative ability of CRC cells was detected by CCK-8 cell proliferation assay and cell clone formation assay. Results: 1) MCM4 is expressed at higher levels in CRC tissues and cells than in normal colorectal tissues and cells. 2) MCM4 promotes the proliferative capacity of colorectal cancer cells. 3) High expression of MCM4 was significantly associated with poor prognosis. Conclusions: MCM4 promotes the development of colorectal cancer and may be an effective colorectal tumor marker and a potential therapeutic target.