β-罗勒烯对烟蚜种群的防控效果

Research on the Control Effect of β-Ocimene on Myzus persicae Population

摘要: 为了探究β-罗勒烯对烟田烟蚜种群的控制效果及最佳施用浓度，在室内条件下分析了不同浓度β-罗勒烯对烟蚜种群数量、种群增长率、干扰作用控制指数以及趋避效果的影响。通过田间药效试验分析了β-罗勒烯及70%吡虫啉水分散粒剂对烟蚜种群数量的影响。室内试验结果表明，10 mM β-罗勒烯对烟蚜种群控制效果最佳，相较于对照，施用β-罗勒烯后12 d每株烤烟上烟蚜密度减少81.7头/株。田间药效试验结果表明，β-罗勒烯能有效抑制田间烟蚜种群数量，综合效果优于化学农药。进一步的RT-qPCR分析结果表明，β-罗勒烯处理能显著提高烤烟植株中水杨酸途径指示基因PR1和茉莉酸/乙烯途径指示基因PDF1.2的表达量，这些说明β-罗勒烯能通过同时激活多条防御途径以促进烤烟植株对烟蚜的防御能力。研究结果表明10 mM β-罗勒烯可用于对烟田烟蚜的控制，本研究为烟田绿色防控烟蚜提供了新的途径。

Abstract: To investigate β-ocimene control effect and optimal application concentration of β-ocimene on the population of Myzus persicae, we investigated the impact of different concentrations of β-ocimene on the population size, population growth rate, repellency rate, and disturbance control index on Myzus persicae was analyzed under laboratory conditions. Under field conditions β-ocimene on the population quantity of Myzus persicae and its impact on the agronomic traits of flue-cured tobacco under the control of β-ocimene. The results are as follows: Through indoor experiments study 10 mM β-ocimene has the best control effect on the Myzus persicae population. Within 12 days, the number of adults decreased by 81.7 heads/plant compared to the control group. The results of the field experiment: Compared to the control β-ocimene can effectively inhibit the population of Myzus persicae in the field. The RT-qPCR analysis results indicate that, β-ocimene treatment with β-ocimene, the expression levels of the salicylic acid pathway marker gene PR1 and the jasmonic acid/ethylene pathway marker gene PDF1.2 in flue-cured tobacco plants were significantly increased, indicating that β-ocimene can promote the defense ability of flue-cured tobacco plants against Myzus persicae by simultaneously activating multiple defense pathways. The research results indicate that 10 mM β-ocimene can be used for controlling Myzus persicae in tobacco fields, our study provides new insights for the prevention and control of Myzus persicae in the field.