血液代谢物水平与慢性鼻窦炎风险之间因果关系：双向两样本孟德尔随机化研究
Causal Relationship between Blood Metabolite Levels and Risk of Chronic Rhinosinusitis: A Two-Way Two-Sample Mendelian Randomization Study
DOI: 10.12677/acm.2024.1451706, PDF,   
作者: 杨 娜：山东省青岛大学医学部，山东 青岛；王冬青, 杨阳洋, 吕怀庆*：临沂市人民医院耳鼻咽喉头颈外科，山东 临沂；陈庆泳*：滨州医学院第二临床医学院，山东 烟台
关键词: 孟德尔随机化；慢性鼻窦炎；血液代谢物；因果关系；Mendelian Randomization； Chronic Sinusitis； Blood Metabolites； Causal Relationship
摘要: 目的：运用孟德尔随机化分析方法，探讨1400种血液代谢物与慢性鼻窦炎之间的因果关系。方法：本研究采用两样本孟德尔随机化分析，利用全基因组关联研究(Genome-wide Association study, GWAS)数据库获得血液代谢物和慢性鼻窦炎相关数据。使用R软件和Two Sample MR软件包进行分析。研究采用逆方差加权法(IVW)为主要分析方法，结合MR-Egger回归、加权中位数法、简单模型法及加权模型法作为补充分析血液代谢物和慢性鼻窦炎的因果关系，并进行异质性分析、多效性分析及敏感性分析评估结果的可靠性和稳定性。由于样本过大，为了结果更加严谨对结果进行错误发现率(FDR)矫正。结果：分析结果显示，发现1种血液代谢物为1-硬脂酰-2-亚油酰-GPI (18:0/18:2)升高与慢性鼻窦炎呈负向因果相关(IVW:OR = 0.873, 95% CI: 0.816~0.934, P < 0.001)，经Cochran Q检验及MR-Egger回归检验，发现不存在水平多效性(P > 0.05)；异质性检验结果P值均<0.001，通过留一法检验分析证实，单个SNPs对整体结果没有显著影响，进一步增强了结果的可靠性和稳定性。结论：在1400种血液代谢物种发现1种1-硬脂酰-2-亚油酰-GPI (18:0/18:2)与慢性鼻窦炎发病存在负向因果关联。通过血液代谢物可为慢性鼻窦炎发病机制及早期预防和治疗提供参考。
Abstract: Objective: To explore the causal relationship between 1400 blood metabolites and chronic sinusitis by using Mendelian randomization analysis method. Methods: Two-sample Mendelian randomization analysis was used in this study, and the data related to blood metabolites and chronic sinusitis were obtained from the Genome-wide Association study (GWAS) database. R software and Two Sample MR software package were used for analysis. The inverse variance weighting method (IVW) was used as the main analysis method, combined with MR-Egger regression, weighted median method, simple model method and weighted model method as supplementary analysis of the causal relationship between blood metabolites and chronic sinusitis, and the reliability and stability of the results were evaluated by heterogeneity analysis, pleiotropy analysis and sensitivity analysis. Due to the large sample size, the results were corrected by false detection rate (FDR) for more rigorous results. Results: The analysis results showed that the increase of 1-stearyl-2-linoleic acid-GPI (18:0/18:2) was negatively correlated with chronic sinusitis (IVW: OR = 0.873, 95% CI: 0.816~0.934, P < 0.001). The Cochran Q test and MR-Egger regression test showed that there was no horizontal pleiotropy (P > 0.05). The results of heterogeneity test were all P < 0.001. The analysis of the one-way ANOVA confirmed that single SNPs had no significant effect on the overall results, further enhancing the reliability and stability of the results. Conclusions: Among 1400 blood metabolites, 1-stearyl-2-linoleic-GPI (18:0/18:2) was found to be negatively correlated with chronic sinusitis, which may provide a reference for the pathogenesis, early prevention and treatment of chronic sinusitis.
文章引用：杨娜, 王冬青, 杨阳洋, 陈庆泳, 吕怀庆. 血液代谢物水平与慢性鼻窦炎风险之间因果关系：双向两样本孟德尔随机化研究[J]. 临床医学进展, 2024, 14(5): 2447-2456. https://doi.org/10.12677/acm.2024.1451706

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