OGT在胰腺癌中表达及预后的相关研究

Study on Expression and Prognosis of OGT in Pancreatic Cancer

摘要: 目的：胰腺癌(Pancreatic cancer, PC)是一种高度侵袭性的恶性肿瘤，对患者的生存率造成严重威胁。越来越多的研究表明，糖基化修饰在胰腺癌的发生和发展中扮演着关键角色。O-连接β-N-乙酰氨基葡萄糖转移酶(O-linked N-acetylglucosamine transferase, OGT)是糖基化的关键调节者，其异常表达与多种癌症相关。本研究旨在探究OGT在胰腺癌中的异常表达，并分析其与胰腺癌预后的相关性。方法：回顾性收集2017年1月至2021年1月就诊于青岛大学附属医院确诊胰腺癌且行手术治疗患者的临床及组织病理资料。临床资料包括患者年龄、性别、身体质量指数(Body mass index, BMI)、烟酒史、糖尿病病史、家族史、肿瘤大小、位置、TNM分期、组织浸润及器官转移情况、癌胚抗原(Carcinoembryonic antigen, CEA)、糖类抗原19-9 (Carbohydrate antigen19-9, CA19-9)水平等信息。应用免疫组织化学染色的方法检测癌组织及癌旁胰腺导管组织中OGT的表达水平，并根据表达量的高低分为高表达组和低表达组，分析其与患者临床病理特征及预后的关系。结果：1) 胰腺癌组织中OGT的表达水平与癌旁组织的表达水平之间存在显著统计学差异(P < 0.001)。2) OGT高表达与淋巴结转移、TNM分期有关(P < 0.05)，与年龄、性别、BMI、吸烟、饮酒情况、糖尿病病史、家族史、肿瘤大小、位置、CEA、CA19-9等因素无相关性(P > 0.05)。3) 生存分析表明，OGT的表达水平与患者不良预后存在明显相关性(P = 0.029)。结论：胰腺癌组织中OGT表达水平明显增加，OGT的高表达与淋巴结转移、TNM分期正相关。胰腺癌中OGT的高表达与胰腺癌患者不良预后相关。

Abstract: Objective: Pancreatic cancer is a highly aggressive tumor that poses a serious threat to the survival rate of patients. A growing body of research suggests that glycosylation regulation plays a key role in the occurrence and progression of pancreatic cancer. O-linked N-acetylglucosamine transferase (OGT) is a key regulator of glycosylation, and its abnormal expression is associated with various types of cancer. Methods: This study collected the clinical and histopathological data of patients who were diagnosed with pancreatic cancer and underwent surgery in the Affiliated Hospital of Qingdao University from January 2017 to January 2021. Information such as age, gender, Body mass index (BMI), smoking, alcohol consumption, diabetes history, family history, tumor size, location, TNM stage, tissue infiltration and organ metastasis, Carcinoembryonic antigen (CEA) and Carbohydrate antigen19-9 (CA19-9) levels were collected. On this basis, we used immunohistochemical staining to detect the expression levels of OGT in cancer tissues and adjacent normal pancreatic duct tissues, and divided them into high expression group and low expression group according to the expression level, and analyzed their correlation with the clinicopathological characteristics and prognosis of patients. Results: 1) There were statistically significant differences between the expression levels of OGT in pancreatic cancer tissues and those in normal tissues adjacent to the cancer (P < 0.001). 2) High expression of OGT was associated with lymph node metastasis and TNM stage (P < 0.05) and there was no correlation with factors such as age, gender, BMI, smoking, alcohol consumption, history of diabetes mellitus, family history, tumour size, location, CEA, CA19-9, etc. correlation (P > 0.05). 3) Survival analysis showed that there was a significant correlation between the expression levels of OGT and the poor prognosis of patients (P = 0.029). Conclusions: The expression levels of OGT were significantly increased in pancreatic cancer tissues. The high expression of OGT was positively correlated with lymph node metastasis and TNM stage. Meanwhile, high expression of OGT was associated with poor prognosis of pancreatic cancer patients.