固肾通督调神法电针对PTSD大鼠行为学的调节作用

Effects of Regulation with Kidney-Strengthening and Du Meridian Regulation Electroacupuncture on Behavioral Changes in Male Rats with PTSD

摘要: 目的：本课题主要研究固肾通督调神法电针早期干预对PTSD雄性大鼠行为学影响。采用改进的单一延长结合足底电击刺激(SPS&S)制作PTSD雄性动物模型，通过旷场箱、Morris水迷宫、电击箱等行为学检测，观察评定大鼠的学习记忆能力、焦虑恐惧情绪、自发活动能力等行为学改变，探寻固肾通督调神法治疗PTSD大鼠行为学上的疗效，为针灸防治PTSD提供理论依据。方法：首先将雄性SD大鼠30只随机分成3组，每组10只，分别采用SPS和SPS&S方法，并运用旷场实验(OF)、高架十字迷宫检测(EPM)和条件恐惧反应实验(CS+)测试进行行为学评估。再将雄性SD大鼠共50只随机分为模型组、西药治疗组、电针治疗组、空白针刺组、空白对照组5组。除空白针刺组和空白对照组，其余动物均接受相同的SPS&S应激进行造模。造模后模型组、空白对照组进行与电针治疗组、空白针刺组相同的固定，但不给予针刺治疗。电针治疗组、空白针刺组进行14天的针刺治疗。14天后，分别进行OF、EPM、CS+和MWM等行为学测验。结果：5组大鼠在治疗后OF测试、EPM测试、MPM测试、闯入性记忆的检测中的评分电针治疗组与模型组均有显著差异(P < 0.01或P < 0.05)，并多数得分与西药治疗组无显著差异(P > 0.05)。闯入性记忆的检测中电针治疗组较西药治疗组警觉、恐惧水平有降低趋势，但无统计学意义(P > 0.05)。四种行为学测试中空白针刺组和空白对照组之间的差异无显著性(P > 0.05)。结论：固肾通督调神法电针可以改善PTSD雌性大鼠的焦虑、恐惧等行为，提高学习记忆能力，作用与帕罗西汀相当。

Abstract: Objective: This study aimed to investigate the behavioral effects of early intervention with kidney-strengthening and du meridian regulation electroacupuncture on male rats with PTSD. An improved single prolonged stress combined with foot shock (SPS&S) was used to establish the PTSD animal model in male rats. Behavioral tests including open field test, Morris water maze, and foot shock box were employed to assess changes in learning and memory, anxiety, fear, and spontaneous activity in rats. The therapeutic effects of kidney-strengthening and du meridian regulation electroacupuncture on PTSD rats were explored to provide theoretical basis for acupuncture treatment of PTSD. Methods: Thirty male SD rats were randomly divided into 3 groups (n = 10 per group) and subjected to SPS and SPS&S methods. Behavioral assessments were conducted using open field test (OF), elevated plus maze (EPM), and conditioned fear response test (CS+). Fifty male SD rats were then randomly divided into model group, Western medicine treatment group, electroacupuncture treatment group, blank acupuncture group, and blank control group. Except for the blank acupuncture group and blank control group, all animals underwent the same SPS&S stress for modeling. After modeling, the model group and blank control group received the same fixation as the electroacupuncture treatment group and blank acupuncture group but without acupuncture treatment. The electroacupuncture treatment group and blank acupuncture group received 14 days of acupuncture treatment. Behavioral tests including OF, EPM, CS+, and Morris water maze (MWM) were conducted after 14 days. Results: There were significant differences between the electroacupuncture treatment group and the model group in the OF test, EPM test, MPM test, and intrusive memory test after treatment (P < 0.01 or P < 0.05), and there was no significant difference in most scores compared to the western medicine treatment group (P > 0.05). In the intrusive memory test, the electroacupuncture treatment group had a trend of decreased vigilance and fear levels compared to the western medicine treatment group, but this was not statistically significant (P > 0.05). There was no significant difference between the blank acupuncture group and the blank control group in the four behavioral tests (P > 0.05). Conclusion: The electroacupuncture treatment of the Gu Shen Tong Duo Shen method can improve the anxiety and fear behaviors of female rats with PTSD and enhance learning and memory abilities, with an effect comparable to paroxetine.