鱼腥草内源抑菌活性物质及其在青枯雷尔氏菌中的应用
Endogenous Antimicrobial Substances in Houttuynia cordata and Their Application against Ralstonia solanacearum
DOI: 10.12677/hjas.2024.146091, PDF,    科研立项经费支持
作者: 张洪郝, 李凌峰, 杨 林, 刘佳欣, 李君竹, 周子豪, 杨 勇, 向海波*：湖北大学生命科学学院，湖北 武汉；黎妍妍*：湖北省烟草科学研究院，湖北 武汉
关键词: 鱼腥草；甲基正壬酮；烟草青枯病；抑菌活性；生物防治；Houttuynia cordata； Methyl Nonyl Ketone； Tobacco Wilt Disease； Antimicrobial Activity；Biological Control
摘要: 本研究聚焦于鱼腥草(Houttuynia cordata Thunb.)内源活性物质在防控烟草雷尔氏菌(Ralstonia solanacearum)中的应用。研究发现，甲基正壬酮(Methyl Nonyl Ketone, MNK)能有效抑制烟草青枯病病原菌的生长。本文系统地描述了MNK的提取、纯化及鉴定流程，并深入分析了其对病原菌生长特性、半抑菌浓度(IC50)、运动性和生物膜形成的影响。实验结果证实了MNK作为植物源活性物质在生物防治烟草青枯病方面的潜力，为病害管理提供了新的策略。
Abstract: This study focuses on the application of endogenous active substances in Houttuynia cordata (Thunb.) for the control and prevention of Ralstonia solanacearum in tobacco. It was found that Methyl Nonyl Ketone (MNK) can effectively inhibit the growth of the pathogen causing tobacco wilt disease. This paper systematically describes the extraction, purification, and identification process of MNK, and deeply analyzes its effects on the growth characteristics, inhibitory concentration 50% (IC50), motility, and biofilm formation of the pathogen. The experimental results confirm the potential of MNK as a plant-derived active substance in the biological control of tobacco wilt disease, providing a new strategy for disease management.
文章引用：张洪郝, 李凌峰, 杨林, 刘佳欣, 李君竹, 周子豪, 杨勇, 向海波, 黎妍妍. 鱼腥草内源抑菌活性物质及其在青枯雷尔氏菌中的应用[J]. 农业科学, 2024, 14(6): 729-739. https://doi.org/10.12677/hjas.2024.146091

参考文献

[1] De Boer, W., Wagenaar, A., Klein Gunnewiek, P.J.A. and Van Veen, J.A. (2007) In vitro Suppression of Fungi Caused by Combinations of Apparently Non-Antagonistic Soil Bacteria. FEMS Microbiology Ecology, 59, 177-185. [Google Scholar] [CrossRef] [PubMed]
[2] Ray, P., Kundu, S. and Paul, D. (2024) Exploring the Therapeutic Properties of Chinese Mushrooms with a Focus on Their Anti-Cancer Effects: A Systemic Review. Pharmacological Research-Modern Chinese Medicine, 11, Article 100433. [Google Scholar] [CrossRef] 
[3] Singh, S. and Verma, R. (2024) Comprehensive Review on Pharmacological Potential of Illicium verum, Chinese Herb. Pharmacological Research-Modern Chinese Medicine, 10, Article 100411. [Google Scholar] [CrossRef] 
[4] Tyagi, P., Prasad, M., Mathur, S. and Ranjan, R. (2024) Diosgenin Biosynthesis Investigation in Medicinal Herb (Tribulus terrestris) by Transcriptome Analysis. Gene, 893, Article 147937. [Google Scholar] [CrossRef] [PubMed]
[5] Fang, Y., Shao, S. and Wang, H. (2018) Intervention Effects of Compound Houttuyniae Herba to Diabetic Renal Damage Based on SOCS-JAK/STAT Negative Feedback Regulation. Chinese Herbal Medicines, 10, 424-430. [Google Scholar] [CrossRef] 
[6] Phosri, S., Kiattisin, K., Intharuksa, A., Janon, R., Na Nongkhai, T. and Theansungnoen, T. (2022) Anti-Aging, Anti-Acne, and Cytotoxic Activities of Houttuynia cordata Extracts and Phytochemicals Analysis by LC-MS/MS. Cosmetics, 9, Article 136. [Google Scholar] [CrossRef] 
[7] Wang, Q.R. and Shao, J. (2022) Chinese Medicinal Formulae Treat Inflammatory Bowel Diseases through Maintaining Gut Flora Homeostasis. China Journal of Chinese Materia Medica, 47, 5997-6004.
[8] Yang, Y., Lai, Q., Wang, C. and Zhou, G. (2022) Protective Effects of Herba Houttuyniae Aqueous Extract against OVA-Induced Airway Hyperresponsiveness and Inflammation in Asthmatic Mice. Evidence-Based Complementary and Alternative Medicine, 2022, Article 7609785. [Google Scholar] [CrossRef] [PubMed]
[9] Ho, T., Lo, H., Lu, G., Liao, P. and Hsiang, C. (2023) Analysis of Target Organs of Houttuynia cordata: A Study on the Anti-Inflammatory Effect of Upper Respiratory System. Journal of Ethnopharmacology, 315, Article 116687. [Google Scholar] [CrossRef] [PubMed]
[10] Tang, J., Zhou, L., Yuan, G., Liu, Y., Shi, X., Lu, Y., et al. (2023) Therapeutic Effects on H1N1-Induced Pneumonia in Mice and Intestinal Bacteria Biotransformation of Four Main Flavonoids from Houttuynia cordata Thunb. Journal of Pharmaceutical and Biomedical Analysis, 233, Article 115469. [Google Scholar] [CrossRef] [PubMed]
[11] Sekita, Y., Murakami, K., Yumoto, H., Hirao, K., Amoh, T., Fujiwara, N., et al. (2017) Antibiofilm and Anti-Inflammatory Activities of Houttuynia cordata Decoction for Oral Care. Evidence-Based Complementary and Alternative Medicine, 2017, Article 2850947. [Google Scholar] [CrossRef] [PubMed]
[12] Ahn, J., Chae, H., Chin, Y. and Kim, J. (2017) Alkaloids from Aerial Parts of Houttuynia cordata and Their Anti-Inflammatory Activity. Bioorganic & Medicinal Chemistry Letters, 27, 2807-2811. [Google Scholar] [CrossRef] [PubMed]
[13] Tans-Kersten, J., Brown, D. and Allen, C. (2004) Swimming Motility, a Virulence Trait of Ralstonia solanacearum, Is Regulated by FlhDC and the Plant Host Environment. Molecular Plant-Microbe Interactions, 17, 686-695. [Google Scholar] [CrossRef] [PubMed]
[14] 杨姗姗, 孙柏欣, 王铁霖, 等. 西瓜嗜酸菌趋化性的初步研究[J]. 植物保护, 2016, 42(3): 161-164, 189.
[15] Peeters, E., Nelis, H.J. and Coenye, T. (2008) Comparison of Multiple Methods for Quantification of Microbial Biofilms Grown in Microtiter Plates. Journal of Microbiological Methods, 72, 157-165. [Google Scholar] [CrossRef] [PubMed]
[16] 张丽, 孙建鹏, 巴其斌. 鱼腥草乙酸乙酯萃取部位的化学成分及抗植物病原菌活性研究[J]. 天然产物研究与开发, 2024, 36(4): 616-621.
[17] 段劲生, 王梅, 胡本进, 等. 中草药源杀菌剂的研究进展[J]. 安徽农业科学, 2007, 35(3): 755, 778. [Google Scholar] [CrossRef] 
[18] 张晓南, 王建豪, 王佳丽, 等. 番石榴叶挥发油的抗菌性及优化提取研究[J]. 热带作物学报, 2023, 44(1): 174-185.

为你推荐