基于三维荧光光谱–平行因子分析法的水中溶解性有机质检测研究

Research on Detection of Aqueous Dissolved Organic Matter in Water Using Three-Dimensional Fluorescence Spectroscopy-Parallel Factor Analytical Method

摘要: 项目以校园内自然河水为研究对象，利用三维荧光光谱技术，结合平行因子法和相关性分析，对水中溶解性有机质(DOM)的组成和变化进行了研究。结果表明：自然河水含有两类3个荧光组分，分别为类腐殖酸荧光组分(C1和C2)和可溶性的微生物副产物荧光组分(C3)。荧光指数(FI)、自生源指数(BIX)、腐殖化指数(HIX)和新鲜度指数(β:α)分别为1.5~1.9、0.8~1.0、0.5~0.7和0.5~1.0，表明水中DOM由陆源输入和内源释放共同造成，水体DOM腐殖化程度较弱。结合相关性分析得出，校园内自然河水组分C3分别与β:α和BIX呈极显著正相关性，这表明在特定条件下组分C3对水体中的β:α和BIX有指示功能。结果表明利用荧光光谱结合平行因子法对水体检测及分析是较快捷、方便的适宜的方法，同时对水体的环境行为起到一定指导作用。

Abstract: Using three-dimensional fluorescence spectroscopy technology, combined with parallel factor analysis and correlation analysis, the composition and variations of Dissolved Organic Matter (DOM) from natural river water on campus were studied. The results showed that the natural river water contained two types of 3 fluorescent components, namely, humic acid-like fluorescent components (C1 and C2) and soluble microbial by-product fluorescent components (C3). The fluorescence index (FI), autogeny index (BIX), humification index (HIX) and freshness index (β:α) were 1.5~1.9, 0.8~1.0, 0.5~0.7 and 0.5~1.0, respectively, indicating that DOM was caused by terrigenous input and endogenous release, and the humification degree of DOM was weak. Combined with the correlation analysis, it was concluded that the C3 in natural river water had extremely significant positive correlation with β:α and BIX, respectively, which indicated that component C3 had an indicator function on β:α and BIX in the water body under certain conditions. The study showed that the fluorescence spectroscopy combined with the parallel factor method is a quick and convenient method for water detection and analysis. Moreover, it plays a guiding role in the water environmental behavior.