电针对便秘型肠易激综合征模型大鼠抑郁样行为及血清IL-4的影响
Effect of Electroacupuncture on Depression-Like Behavior and Serum IL-4 in Rats with Constipated Irritable Bowel Syndrome Model
摘要: 目的：观察电针对便秘型肠易激综合征(IBS-C)模型大鼠抑郁样行为及血清IL-4的影响，探讨电针改善便秘型肠易激综合征抑郁样行为的机制。方法：采用冰水灌胃法制备IBS-C模型大鼠，造模成功后随机分为模型对照组、电针干预组、手针干预组、温针干预组，另设空白对照组，每组5只。电针干预组、手针干预组、温针干预组分别给予“足三里”、“天枢”、“三阴交”组合电针、手针、温针等干预措施，持续7天，干预结束后评估各组大鼠的一般情况和体重变化，利用强迫游泳实验(FST)和糖水偏好实验(SPT)检测各组大鼠抑郁行为学表现，利用酶联免疫吸附试验(ELISA)检测各组大鼠血清中IL-4含量。结果：与空白对照组相比，模型组大鼠AWR评分显著升高(P < 0.05)，表明IBS-C大鼠模型制备成功。干预7天后，与模型对照组相比，电针干预组、手针干预组、温针干预组大鼠干预后体重，体重差值明显升高(P < 0.05)，抑郁样行为得到显著改善(P < 0.05)，血清中IL-4含量显著升高(P < 0.05)，以电针组为著。结论：电针治疗可以显著改善IBS-C大鼠抑郁行为学表现，对于IBS-C康复治疗具有重要的临床意义，其作用机制可能与调节IBS-C模型大鼠炎症因子IL-4的水平有关。
Abstract: Objective: To observe the effects of electroacupuncture on depression-like behavior and serum IL-4 in constipated irritable bowel syndrome (IBS-C) model rats, and to explore the mechanism of electroacupuncture to improve the depression-like behavior of constipated irritable bowel syndrome. Method: IBS-C model rats were prepared by ice-water gavage, and randomly divided into model control group, electroacupuncture intervention group, hand acupuncture intervention group, warm acupuncture intervention group, and blank control group, with 5 rats in each group after successful modeling. The electroacupuncture intervention group, hand-acupuncture intervention group, and warm-acupuncture intervention group were given a combination of electroacupuncture, hand-acupuncture and warm-acupuncture measures, such as “foot-sanli”, “tian shu”, and “san yin jiao”, respectively, for 7 days, and the rats were evaluated in each group at the end of the intervention. At the end of the intervention, the general condition and body weight of the rats in each group were evaluated, and the behavioral manifestations of depression were detected using the forced swimming test (FST) and the sugar-water preference test (SPT), and the serum levels of IL-4 in each group were detected using the enzyme-linked immunosorbent assay (ELISA). Result: Compared with the blank control group, the AWR scores of rats in the model group were significantly higher (P < 0.05), indicating that the IBS-C rat model was successfully prepared. After 7 days of intervention, compared with the model control group, rats in the electroacupuncture intervention group, hand-acupuncture intervention group, and warm-acupuncture intervention group had significantly higher body weights, body weight difference (P < 0.05), significantly improved depression-like behaviors (P < 0.05), and significantly higher serum levels of IL-4 (P < 0.05) after the intervention, with the electroacupuncture group being the most prominent. Conclusion: Electroacupuncture treatment can significantly improve the behavioral manifestations of depression in IBS-C rats, which is of great clinical significance for the rehabilitation treatment of IBS-C, and its mechanism of action may be related to the regulation of the level of inflammatory factor IL-4 in IBS-C model rats.
文章引用：冯敏, 何婷, 古巧燕. 电针对便秘型肠易激综合征模型大鼠抑郁样行为及血清IL-4的影响[J]. 临床医学进展, 2024, 14(7): 673-681. https://doi.org/10.12677/acm.2024.1472066

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