新型冠状病毒SARS-CoV-2检测技术研究进展
Progress in Detection of Novel Coronavirus SARS-CoV-2
DOI: 10.12677/acm.2024.1472112, PDF,    科研立项经费支持
作者: 王熙函#, 赵 藤#, 魏小丽#, 周彩红, 赵梓芊, 殷利眷*：天津科技大学生物工程学院，食品营养与安全教育部重点实验室，工业发酵微生物教育部重点实验室，天津；卫春会：酿酒生物技术及应用四川省重点实验室，四川 宜宾；邓冉冉*：邯郸市第一医院妇产科，河北 邯郸
关键词: 新型冠状病毒；COVID-19；检测；CRISPR/Cas；SARS-COV-2； COVID-19； Detection； CRISPR/Cas
摘要: 由新型冠状病毒(SARS-CoV-2)导致的新型冠状病毒感染仍是全世界面临的重要公共卫生事件，且随着病毒基因组不断发生变异，新型变异株出现，病毒传染性和传播能力急剧增强，对人类健康造成严重威胁。快速、高灵敏、高特异、即时检测新型冠状病毒对疫情防控和疾病快速诊治具有十分重要意义。本文基于国内外相关研究文献，对目前新型冠状病毒检测方法进行总结和梳理，包括实时定量RT-PCR、等温核酸扩增技术、CRISPR-Cas核酸检测技术以及高通量测序等核酸检测方法以及酶联免疫吸附法、侧流免疫层析等免疫学方法。进一步，对不同的新型冠状病毒检测方法进行分析和讨论，以期为新型冠状病毒检测和疫情防控提供方法参考。
Abstract: Novel coronavirus infection caused by the new coronavirus (SARS-CoV-2) is still an important public health event facing the world, and with the continuous mutation of the virus genome and the emergence of new variants, the infectivity and transmissibility of the virus have increased dramatically, posing a serious threat to human health. Rapid, highly sensitive, highly specific, and real-time detection of the novel coronavirus is of great significance for epidemic prevention and control, rapid diagnosis and treatment of diseases. Based on relevant research literature at home and abroad, this paper summarizes and sorts out the current research progress of novel coronavirus detection methods, including real-time RT-PCR, isothermal amplification nucleic acid detection technology, CRISPR-Cas nucleic acid detection technology, high-throughput sequencing and other nucleic acid detection methods, as well as immunological methods such as enzyme-linked immunosorbent detection and antigen colloidal gold test strip. Furthermore, different detection methods were analyzed and compared in order to provide a reference for novel coronavirus detection and epidemic prevention and control.
文章引用：王熙函, 赵藤, 魏小丽, 周彩红, 赵梓芊, 卫春会, 邓冉冉, 殷利眷. 新型冠状病毒SARS-CoV-2检测技术研究进展[J]. 临床医学进展, 2024, 14(7): 1041-1049. https://doi.org/10.12677/acm.2024.1472112

参考文献

[1] 周烨真, 张世豪, 陈嘉仪, 等. 新型冠状病毒SARS-CoV-2的变异和进化分析[J]. 南方医科大学学报, 2020, 40(2): 152-158.
[2] 罗晓君, 章文贤. 冠状病毒的结构及生物学特性概述[J]. 生物学教学, 2020, 45(7): 4-6.
[3] Tao, K., Tzou, P.L., Nouhin, J., Gupta, R.K., de Oliveira, T., Kosakovsky Pond, S.L., et al. (2021) The Biological and Clinical Significance of Emerging SARS-CoV-2 Variants. Nature Reviews Genetics, 22, 757-773. [Google Scholar] [CrossRef] [PubMed]
[4] Liu, Y., Wang, Y., Wang, X., Xiao, Y., Chen, L., Guo, L., et al. (2020) Development of Two Taqman Real-Time Reverse Transcription-PCR Assays for the Detection of Severe Acute Respiratory Syndrome Coronavirus-2. Biosafety and Health, 2, 232-237. [Google Scholar] [CrossRef] [PubMed]
[5] Merindol, N., Pépin, G., Marchand, C., Rheault, M., Peterson, C., Poirier, A., et al. (2020) SARS-CoV-2 Detection by Direct rRT-PCR without RNA Extraction. Journal of Clinical Virology, 128, Article ID: 104423. [Google Scholar] [CrossRef] [PubMed]
[6] Vogels, C.B.F., Brito, A.F., Wyllie, A.L., Fauver, J.R., Ott, I.M., Kalinich, C.C., et al. (2020) Analytical Sensitivity and Efficiency Comparisons of SARS-CoV-2 RT-qPCR Primer-Probe Sets. Nature Microbiology, 5, 1299-1305. [Google Scholar] [CrossRef] [PubMed]
[7] Tahan, S., Parikh, B.A., Droit, L., Wallace, M.A., Burnham, C.D. and Wang, D. (2021) SARS-CoV-2 E Gene Variant Alters Analytical Sensitivity Characteristics of Viral Detection Using a Commercial Reverse Transcription-PCR Assay. Journal of Clinical Microbiology, 59, e00075-21. [Google Scholar] [CrossRef] [PubMed]
[8] Notomi, T. (2000) Loop-Mediated Isothermal Amplification of DNA. Nucleic Acids Research, 28, 63e. [Google Scholar] [CrossRef] [PubMed]
[9] Dao Thi, V.L., Herbst, K., Boerner, K., Meurer, M., Kremer, L.P., Kirrmaier, D., et al. (2020) A Colorimetric RT-LAMP Assay and Lamp-Sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples. Science Translational Medicine, 12, eabc7075. [Google Scholar] [CrossRef] [PubMed]
[10] Rabe, B.A. and Cepko, C. (2020) SARS-CoV-2 Detection Using Isothermal Amplification and a Rapid, Inexpensive Protocol for Sample Inactivation and Purification. Proceedings of the National Academy of Sciences of the United States of America, 117, 24450-24458. [Google Scholar] [CrossRef] [PubMed]
[11] Piepenburg, O., Williams, C.H., Stemple, D.L. and Armes, N.A. (2006) DNA Detection Using Recombination Proteins. PLOS Biology, 4, e204. [Google Scholar] [CrossRef] [PubMed]
[12] 张淼源, 卢佩珊, 李佳宁, 等. 重组酶聚合酶侧流层析技术检测新冠病毒核酸快速诊断方法的初步建立[J]. 中国人兽共患病学报, 2022, 38(7): 577-581.
[13] Xia, S. and Chen, X. (2020) Single-Copy Sensitive, Field-Deployable, and Simultaneous Dual-Gene Detection of SARS-CoV-2 RNA via Modified RT-RPA. Cell Discovery, 6, Article No. 37. [Google Scholar] [CrossRef] [PubMed]
[14] Harrison, M.M., Jenkins, B.V., O’Connor-Giles, K.M. and Wildonger, J. (2014) A CRISPR View of Development. Genes & Development, 28, 1859-1872. [Google Scholar] [CrossRef] [PubMed]
[15] Gootenberg, J.S., Abudayyeh, O.O., Lee, J.W., Essletzbichler, P., Dy, A.J., Joung, J., et al. (2017) Nucleic Acid Detection with CRISPR-Cas13a/C2c2. Science, 356, 438-442. [Google Scholar] [CrossRef] [PubMed]
[16] Chen, J.S., Ma, E., Harrington, L.B., Da Costa, M., Tian, X., Palefsky, J.M., et al. (2018) CRISPR-Cas12a Target Binding Unleashes Indiscriminate Single-Stranded DNase Activity. Science, 360, 436-439. [Google Scholar] [CrossRef] [PubMed]
[17] Li, Y., Li, S., Wang, J. and Liu, G. (2019) CRISPR/Cas Systems towards Next-Generation Biosensing. Trends in Biotechnology, 37, 730-743. [Google Scholar] [CrossRef] [PubMed]
[18] Broughton, J.P., Deng, X., Yu, G., Fasching, C.L., Servellita, V., Singh, J., et al. (2020) CRISPR/Cas 12-Based Detection of SARS-CoV-2. Nature Biotechnology, 38, 870-874. [Google Scholar] [CrossRef] [PubMed]
[19] Joung, J., Ladha, A., Saito, M., Kim, N., Woolley, A.E., Segel, M., et al. (2020) Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing. New England Journal of Medicine, 383, 1492-1494. [Google Scholar] [CrossRef] [PubMed]
[20] Ma, L., Yin, L., Li, X., Chen, S., Peng, L., Liu, G., et al. (2022) A Smartphone-Based Visual Biosensor for CRISPR-Cas Powered SARS-CoV-2 Diagnostics. Biosensors and Bioelectronics, 195, Article ID: 113646. [Google Scholar] [CrossRef] [PubMed]
[21] Maturada, P., Krittapas, J., Archiraya, P., et al. (2020) Clinical Validation of a Cas13-Based Assay for the Detection of SARS-CoV-2 RNA. Nature Biomedical Engineering, 4, 1140-1149.
[22] Arizti-Sanz, J., Bradley, A., Zhang, Y.B., Boehm, C.K., Freije, C.A., Grunberg, M.E., et al. (2022) Simplified Cas13-Based Assays for the Fast Identification of SARS-CoV-2 and Its Variants. Nature Biomedical Engineering, 6, 932-943. [Google Scholar] [CrossRef] [PubMed]
[23] Yin, L., Man, S., Ye, S., Liu, G. and Ma, L. (2021) CRISPR-Cas Based Virus Detection: Recent Advances and Perspectives. Biosensors and Bioelectronics, 193, Article ID: 113541. [Google Scholar] [CrossRef] [PubMed]
[24] Lu, R., Zhao, X., Li, J., et al. (2020) Genomic Characterisation and Epidemiology of 2019 Novel Coronavirus: Implications for Virus Origins and Receptor Binding. The Lancet, 395, 565-574.
[25] Zhou, P., Yang, X., Wang, X., Hu, B., Zhang, L., Zhang, W., et al. (2020) A Pneumonia Outbreak Associated with a New Coronavirus of Probable Bat Origin. Nature, 579, 270-273. [Google Scholar] [CrossRef] [PubMed]
[26] Wang, L. and Cheng, G. (2021) Sequence Analysis of the Emerging Sars‐cov‐2 Variant Omicron in South Africa. Journal of Medical Virology, 94, 1728-1733. [Google Scholar] [CrossRef] [PubMed]
[27] Wang, M., Fu, A., Hu, B., Tong, Y., Liu, R., Liu, Z., et al. (2020) Nanopore Targeted Sequencing for the Accurate and Comprehensive Detection of SARS‐CoV‐2 and Other Respiratory Viruses. Small, 16, e2002169. [Google Scholar] [CrossRef] [PubMed]
[28] Engvall, E. (2010) The ELISA, Enzyme-Linked Immunosorbent Assay. Clinical Chemistry, 56, 319-320. [Google Scholar] [CrossRef] [PubMed]
[29] Liu, W., Liu, L., Kou, G., Zheng, Y., Ding, Y., Ni, W., et al. (2020) Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2. Journal of Clinical Microbiology, 58, e00461-20. [Google Scholar] [CrossRef] [PubMed]
[30] Li, Z., Yi, Y., Luo, X., Xiong, N., Liu, Y., Li, S., et al. (2020) Development and Clinical Application of a Rapid IgM‐IgG Combined Antibody Test for SARS‐CoV‐2 Infection Diagnosis. Journal of Medical Virology, 92, 1518-1524. [Google Scholar] [CrossRef] [PubMed]
[31] Serebrennikova, K.V., Byzova, N.A., Zherdev, A.V., Khlebtsov, N.G., Khlebtsov, B.N., Biketov, S.F., et al. (2021) Lateral Flow Immunoassay of Sars-Cov-2 Antigen with SERS-Based Registration: Development and Comparison with Traditional Immunoassays. Biosensors, 11, Article 510. [Google Scholar] [CrossRef] [PubMed]
[32] Long, Q., Liu, B., Deng, H., Wu, G., Deng, K., Chen, Y., et al. (2020) Antibody Responses to SARS-CoV-2 in Patients with COVID-19. Nature Medicine, 26, 845-848. [Google Scholar] [CrossRef] [PubMed]
[33] Cai, X., Chen, J., li Hu, J., Long, Q., Deng, H., Liu, P., et al. (2020) A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019. The Journal of Infectious Diseases, 222, 189-193. [Google Scholar] [CrossRef] [PubMed]
[34] Lin, D., Liu, L., Zhang, M., Hu, Y., Yang, Q., Guo, J., et al. (2020) Evaluations of the Serological Test in the Diagnosis of 2019 Novel Coronavirus (SARS-CoV-2) Infections during the COVID-19 Outbreak. European Journal of Clinical Microbiology & Infectious Diseases, 39, 2271-2277. [Google Scholar] [CrossRef] [PubMed]
[35] Santos, S.A.S.B., Cunha, R.L.J., Carvalho, C.I., et al. (2022) Nanotechnology Meets Immunology towards a Rapid Diagnosis Solution: The COVID-19 Outbreak Challenge. RSC Advances, 12, 31711-31728. [Google Scholar] [CrossRef] [PubMed]

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