ZNF268在肝细胞癌中的表达及临床诊断价值探讨
The Expression and Clinical Diagnostic Value of ZNF268 in Hepatocellular Carcinoma
DOI: 10.12677/acm.2024.1472137, PDF,    科研立项经费支持
作者: 吴坤亮, 王能乙, 刘 瑞*:海南医科大学第二附属医院感染病与热带病科,海南 海口;郑 根:海南医科大学第二附属医院肾内科,海南 海口
关键词: ZNF268肝细胞癌临床诊断ZNF268 HCC Clinical Diagnosis
摘要: 目的:分析ZNF268在肝细胞癌(HCC)与正常肝脏中的表达差异,探讨ZNF268在HCC中的临床诊断价值。方法:实时荧光定量PCR法(qRT-PCR)分别检测人肝癌细胞株HepG2、Huh7与人正常肝细胞株LO2中ZNF268 mRNA的表达情况;免疫组织化学技术(IHC)检测64例HCC及其癌旁对照组织中ZNF268的表达情况。结果:人肝癌细胞株HepG2、Huh7中ZNF268 mRNA的表达均明显低于人正常肝细胞株LO2 (P < 0.0001, P < 0.0001);HCC组织中ZNF268的表达明显低于癌旁组织(P < 0.05)。结论:ZNF268在HCC中的表达低于正常肝脏,ZNF268有望作为HCC鉴别诊断和治疗的潜在生物标志物。
Abstract: Objective: To analyze the differential expression of ZNF268 in Hepatocellular carcinoma (HCC) and normal liver, and to explore the value of ZNF268 in the clinical diagnosis of HCC. Methods: qRT-PCR was used to detect the expression of ZNF268 mRNA in HepG2, Huh7 and LO2, respectively; IHC was used to detect the expression of ZNF268 in 64 HCC and paracancerous tissues. Results: The expression of ZNF268 mRNA in HepG2 and Huh7 was significantly lower than that in LO2 (P < 0.0001, P < 0.0001); The expression of ZNF268 in HCC tissue was significantly lower than that in paracancerous tissues (P < 0.05). Conclusion: The expression of ZNF268 in HCC is lower than that in normal liver, making it a potential biomarker for differential diagnosis and treatment of HCC.
文章引用:吴坤亮, 郑根, 王能乙, 刘瑞. ZNF268在肝细胞癌中的表达及临床诊断价值探讨[J]. 临床医学进展, 2024, 14(7): 1235-1240. https://doi.org/10.12677/acm.2024.1472137

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