农杆菌介导DR1372基因转化甘蓝型油菜
Preliminary Study on Agrobacterium-Mediated Transformation of DR1372 Gene into Brassica napus L.
DOI: 10.12677/BR.2013.21007, PDF, HTML, XML,    国家自然科学基金支持
作者: 张思维, 周文波, 张 新, 陈翠娜, 代其林：西南科技大学生命科学与工程学院，绵阳；王 劲：中国农业科学院生物技术研究所，北京
关键词: DR1372；油菜；农杆菌介导；遗传转化； DR1372; Brassica napus L.; Genetic Transformation; Drought-Tolerance
摘要:

DR1372基因是在耐辐射奇球菌(Deinococcus radiodurans, DR)基因组中克隆得到的一个基因，其蛋白序列存在一个WHy功能域，此功能域可能参与油菜的抗旱过程。本研究首先构建植物DR1372- pBI121表达载体，然后利用农杆菌介导法将目的基因DR1372成功转入甘蓝型油菜中，并对影响油菜再生频率以及遗传转化效率的几个因素进行了探讨，初步建立了油菜转DR1372基因遗传转化体系，为DR1372基因的功能研究奠定了基础。

Abstract: The DR1372 containing a special domain called WHy was cloned from Deinococcus radiodurans. This WHy domain may be involved in the mechanism of drought resistance. In this study, the plant expression vector DR1372-pBI121 was constructed and introduced into Brassica napus L. mediated by Agrobacteria. Several factors affecting genetic transformation efficiency were explored and the Agrobacterium-mediated genetic transformation system was set up in Brassica napus. This study provides essential materials for later research on DR1372 gene.

文章引用：张思维, 周文波, 张新, 陈翠娜, 代其林, 王劲. 农杆菌介导DR1372基因转化甘蓝型油菜[J]. 植物学研究, 2013, 2(1): 40-45. http://dx.doi.org/10.12677/BR.2013.21007

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