绞股蓝总皂苷提取和纯化工艺的优化

Process Optimization of Extraction and Purification on Gypenosides

摘要: 以五叶绞股蓝的梗和叶、七叶绞股蓝的叶为材料，用水提法和微波干辅助醇提法从中提取了绞股蓝总皂苷，比较了其提取物中总皂苷含量的差异，并分别用HP20大孔吸附树脂柱层析法和石油醚萃取法对提取的绞股蓝总皂苷进行了纯化，比较了两种纯化方法的效果。结果表明七叶绞股蓝叶提取物中的总皂苷含量最高，五叶绞股蓝叶次之，五叶绞股蓝梗最低；微波干辅助醇提的效率高于水提法；本实验中微波醇提–石油醚萃取纯化七叶绞股蓝叶提取物得到的样品中总皂苷的含量最高为89.76%；水提-HP20柱层析法纯化七叶绞股蓝叶提取物得到的样品中总皂苷的含量为76.40%。

Abstract: Gypenosides were extracted from the leaf and stems of five-leave gynostemma and the leaf of seven-leave gynostemma using water extraction and microwave-assisted alcohol extraction. Meanwhile, gypenosides content’s dif- ferences were compared among them, and then the extracted gypenosides were purified by HP20 macroporous resin column chromatography and petroleum ether extraction, the effects of two purification methods were compared. The results show that the gypenosides’ content of the leaf of seven-leave gynostemma was the most, the leaf of five-leave gynostemma followed and the stems of five-leave gynostemma pentaphyllum was the least. Microwave-assisted alcohol extraction is more efficient than the water extraction method. Water extracted gypenosides and purified by HP20 col- umn chromatography is higher than the efficiency of petroleum ether extraction. In this study, the gypenosides’ content from the leaf of seven-leave gynostemma by microwave-assisted ethanol extraction-petroleum ether extraction purified is the most, up to 89.76%; the gypenosides’ content from the leaf of seven-leave gynostemma by water extraction-HP20 column chromatography is 76.40%.