UPLC-PDA测定电子烟烟液中4种人工甜味剂

Determination of Four Types of Artificial Sweeteners in Electronic Cigarette Liquids by UPLC-PDA

摘要: 目的：建立了一种采用超高效液相色谱(UPLC)和二极管阵列检测器(PDA)同时检测电子烟烟液中4种人工高倍甜味剂(糖精钠、阿斯巴甜、爱德万甜、纽甜)的方法。方法：采用Waters ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm)为分析柱，以乙腈-0.02 mol/L醋酸铵水溶液(pH为4.5)为流动相，梯度洗脱，流速为0.4 mL/min，温度为35℃，外标法定量。结果：4种人工甜味剂在5~100 μg/mL浓度范围内线性良好，相关系数均>0.9990。方法定量限为26.8~212.7 mg/kg，平均加样回收率为93.6%~108.3%。运用此方法对10种不同风味的电子烟液进行检测。除烟草味外，多数电子液体中都含有人工甜味剂，纽甜出现频率最高。结论：该方法具有灵敏、准确、有效、方便等优点，适用于电子烟烟液中人工甜味剂的检测。

Abstract: Objective: A method of ultra-high performance liquid chromatography (UPLC) and photo-diode array (PDA) was established for the simultaneous determination of four types of artificial high-frequency sweeteners (saccharin sodium, aspartame, advantame, and neotame) in electronic cigarette liquids. Methods: Waters ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm) was used as the analytical column, with acetonitrile-0.02 mol/L ammonium acetate aqueous solution (pH 4.5) as the mobile phase in a gradient elution at a flow rate of 0.4 mL/min and temperature of 35˚C, and quantified by external standard method. Results: The linearity of the four types of artificial sweeteners was good in the concentration range of 5~100 mg/L, and correlation coefficients were all higher than 0.999. The quantitative limit of the method was 26.8~212.7 mg/kg, and the average sample recovery rate was 93.6%~108.3%. This method was used to detect 10 different flavors of electronic cigarette liquids. Except for the tobacco flavor, most electronic liquids contained artificial sweeteners, and neotame was the most frequent. Conclusion: This method is sensitive, accurate, efficient, and convenient, and is suitable for the detection of artificial sweeteners in electronic cigarette liquids.