微生物在雪茄烟叶发酵中的应用研究进展
Research Progress on the Application of Microorganisms in Cigar Tobacco Fermentation
DOI: 10.12677/hjas.2024.1411149, PDF,   
作者: 张玉芹, 王 杰, 周 永, 李军民, 曹长代, 崔丙慧, 薛仁喜, 董世峰, 韩守栋:山东日照烟草有限公司,山东 日照;门奎富, 侯 欣*:山东农业大学植物保护学院,山东 泰安
关键词: 雪茄烟叶发酵化学成分微生物技术Cigar Tobacco Leaves Fermentation Chemical Component Microbial Technology
摘要: 微生物技术在雪茄烟叶发酵过程中作为有效手段,能够促进烟叶化学成分的转化,加速发酵进程,改善烟叶品质。本文分析了雪茄发酵机理,重点探究了微生物群落演替对雪茄品质的影响,提出了目前存在的问题,并探讨了今后微生物应用发展前景,为微生物技术在雪茄发酵中的应用提供参考。
Abstract: Microbial technology, as an effective means in the fermentation process of cigar tobacco leaves, can promote the transformation of chemical components in tobacco leaves, accelerate the fermentation process, and improve the quality of tobacco leaves. This article analyzes the fermentation mechanism of cigars, focuses on exploring the impact of microbial community succession on cigar quality, identifies current problems, and explores the future development prospects of microbial applications, providing reference for the application of microbial technology in cigar fermentation.
文章引用:张玉芹, 王杰, 周永, 李军民, 曹长代, 崔丙慧, 薛仁喜, 董世峰, 韩守栋, 门奎富, 侯欣. 微生物在雪茄烟叶发酵中的应用研究进展[J]. 农业科学, 2024, 14(11): 1178-1184. https://doi.org/10.12677/hjas.2024.1411149

参考文献

[1] 金敖熙. 雪茄烟生产技术[M]. 北京: 轻工业出版社, 1982.
[2] 王浩雅, 左兴俊, 孙福山, 等. 雪茄烟外包叶的研究进展[J]. 中国烟草科学, 2009, 30(5): 71-76.
[3] 刘国顺. 烟草栽培学[M]. 北京: 中国农业出版社, 2003.
[4] 蔡斌, 耿召良, 高华军, 等. 国产雪茄原料生产技术研究现状[J]. 中国烟草学报, 2019, 25(6): 110-119.
[5] 田丽君, 刘佳琪, 单玉静, 等. 微生物在雪茄烟叶发酵中的应用研究进展[J]. 农技服务, 2024, 41(8): 30-36.
[6] 周锦龙, 汤珍瑶. 雪茄烟发酵技术进展与展望[J]. 农技服务, 2009, 26(11): 119-120+127.
[7] 陈茏. 产蛋白酶菌株的筛选及酶学性质的研究[D]: [硕士学位论文]. 南昌: 南昌大学, 2020.
[8] 郭文龙. 茄芯烟叶发酵过程中颜色参数变化及外源加酶发酵对其质量的影响[D]: [硕士学位论文]. 郑州: 河南农业大学, 2022.
[9] 陈宇. 海南雪茄烟发酵过程中的烟叶质量变化研究[D]: [硕士学位论文]. 郑州: 河南农业大学, 2019.
[10] 谭舒, 曾旸, 曾剑平, 等. 发酵对雪茄烟叶品质影响的研究进展[J]. 安徽农业科学, 2023, 51(18): 16-19+28.
[11] Zheng, T., Zhang, Q., Li, P., Wu, X., Liu, Y., Yang, Z., et al. (2022) Analysis of Microbial Community, Volatile Flavor Compounds, and Flavor of Cigar Tobacco Leaves from Different Regions. Frontiers in Microbiology, 13, Article ID: 907270. [Google Scholar] [CrossRef] [PubMed]
[12] Zhong, W., Zhu, C., Shu, M., Sun, K., Zhao, L., Wang, C., et al. (2010) Degradation of Nicotine in Tobacco Waste Extract by Newly Isolated Pseudomonas Sp. Zutskd. Bioresource Technology, 101, 6935-6941. [Google Scholar] [CrossRef] [PubMed]
[13] 周冀衡, 朱小平, 王彦亭, 等. 烟草生理与生物化学[M]. 合肥: 中国科学技术大学出版社, 1996.
[14] 王能如. 烟叶调制与分级[M]. 合肥: 中国科学技术大学出版社, 2002.
[15] 钟庆辉. 烟草化学基本知识[M]. 北京: 轻工业出版社, 1985.
[16] 姚芳. 海南茄衣叶面微生物鉴定及其在人工发酵过程对雪茄烟叶品质的影响[D]: [硕士学位论文]. 郑州: 河南农业大学, 2017.
[17] 刘春奎, 刘春玲, 王国良, 等. 烟叶发酵研究进展[J]. 广西轻工业, 2011, 27(11): 15-16.
[18] 胡婉蓉, 李东亮. 国产雪茄烟叶关键生产技术研究进展[J]. 中国烟草学报, 2024: 1-15.
[19] 朱大恒, 王浩, 韩锦峰, 周国友. 烤烟发酵过程中氧气条件对烟叶化学成分的影响[J]. 河南农业科学, 1999(9): 12-14.
[20] 郑霖霖. 不同发酵工艺下茄芯烟叶的微生物群落与品质变化及相关性分析[D]: [硕士学位论文]. 郑州: 河南农业大学, 2023.
[21] 万德建, 吴创, 杜佳, 等. 雪茄烟叶发酵方法研究进展[J]. 山西农业科学, 2017, 45(7): 1211-1214.
[22] 杨虹琦, 周冀衡, 罗泽民, 梁志怀. 微生物和酶在烟叶发酵中的应用[J]. 湖南农业科学, 2004(1): 63-66.
[23] 李宁, 曾代龙, 戴亚, 等. 雪茄烟叶叶面可培养微生物分离鉴定[J]. 安徽农业科学, 2009, 37(25): 11857-11858.
[24] Hu, W., Cai, W., Jia, Y., Fan, J., Zhu, B., Zhang, Q., et al. (2023) Sensory Attributes, Chemical and Microbiological Properties of Cigars Aged with Different Media. Frontiers in Bioengineering and Biotechnology, 11, Article ID: 1294667. [Google Scholar] [CrossRef] [PubMed]
[25] 张鸽, 李志豪, 邓帅军, 等. 海南H382雪茄烟叶不同发酵周期细菌群落多样性表征及演替分析[J]. 中国烟草学报, 2021, 27(2): 117-126.
[26] 张晓娟. 雪茄外包皮烟人工发酵工艺及叶表微生物区系研究[D]: [硕士学位论文]. 郑州: 河南农业大学, 2006.
[27] Liu, F., Wu, Z., Zhang, X., Xi, G., Zhao, Z., Lai, M., et al. (2021) Microbial Community and Metabolic Function Analysis of Cigar Tobacco Leaves during Fermentation. MicrobiologyOpen, 10, e1171. [Google Scholar] [CrossRef] [PubMed]
[28] 赵铭钦, 邱立友, 张维群, 齐伟成, 岳雪梅. 陈化期间烤烟叶片中生物活性变化的研究[J]. 华中农业大学学报, 2000, 19(6): 537-542.
[29] 吴丽君, 张鸽, 陈晓娜, 等. 云南雪茄烟叶人工发酵过程中化学成分与细菌群落变化及其相关性分析[J]. 中国烟草学报, 2023, 29(4): 124-134.
[30] Li, J., Zhao, Y., Qin, Y. and Shi, H. (2020) Influence of Microbiota and Metabolites on the Quality of Tobacco during Fermentation. BMC Microbiology, 20, Article No. 356. [Google Scholar] [CrossRef] [PubMed]
[31] Zhang, Q., Zheng, T., Yang, Z., Yang, S., Cai, W., Li, P., et al. (2023) Analysis of the Structure and Metabolic Function of Microbial Community in Cigar Tobacco Leaves in Agricultural Processing Stage. Frontiers in Microbiology, 14, Article ID: 1230547. [Google Scholar] [CrossRef] [PubMed]
[32] 郑霖霖, 赵亮, 蔡兴华, 等. 雪茄茄芯烟叶工业二次发酵过程中细菌与真菌群落多样性变化分析[J]. 中国烟草学报, 2022, 28(5): 121-128.
[33] Wu, Q., Peng, Z., Pan, Y., Liu, L., Li, L., Zhang, J., et al. (2023) Interaction Analysis of Tobacco Leaf Microbial Community Structure and Volatiles Flavor Compounds during Cigar Stacking Fermentation. Frontiers in Microbiology, 14, Article ID: 1168122. [Google Scholar] [CrossRef] [PubMed]
[34] Mokashe, N., Chaudhari, A. and Patil, U. (2015) Optimal Production and Characterization of Alkaline Protease from Newly Isolated Halotolerant Jeotgalicoccus Sp. Biocatalysis and Agricultural Biotechnology, 4, 235-243. [Google Scholar] [CrossRef
[35] Arias, R.S., Dang, P.M. and Sobolev, V.S. (2015) RNAi-Mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem. Journal of Visualized Experiments, 106, e53398. [Google Scholar] [CrossRef] [PubMed]
[36] Wu, X., Hu, Y., Wang, Q., Liu, J., Fang, S., Huang, D., et al. (2023) Study on the Correlation between the Dominant Microflora and the Main Flavor Substances in the Fermentation Process of Cigar Tobacco Leaves. Frontiers in Microbiology, 14, Article ID: 1267447. [Google Scholar] [CrossRef] [PubMed]
[37] Stubbs, J. (2010) El Habano and the World It Has Shaped: Cuba, Connecticut, and Indonesia. Cuban Studies, 41, 39-67. [Google Scholar] [CrossRef
[38] Banožić, M., Jokić, S., Ačkar, Đ., Blažić, M. and Šubarić, D. (2020) Carbohydrates—Key Players in Tobacco Aroma Formation and Quality Determination. Molecules, 25, Article No. 1734. [Google Scholar] [CrossRef] [PubMed]
[39] Liu, S., Hu, J., Xu, Y., Xue, J., Zhou, J., Han, X., et al. (2020) Combined Use of Single Molecule Real-Time DNA Sequencing Technology and Culture-Dependent Methods to Analyze the Functional Microorganisms in Inoculated Raw Wheat Qu. Food Research International, 132, Article ID: 109062. [Google Scholar] [CrossRef] [PubMed]
[40] Zhang, L., Mai, J., Shi, J., Ai, K., He, L., Zhu, M., et al. (2024) Study on Tobacco Quality Improvement and Bacterial Community Succession during Microbial Co-fermentation. Industrial Crops and Products, 208, Article ID: 117889. [Google Scholar] [CrossRef
[41] Zheng, T., Zhang, Q., Wu, Q., Li, D., Wu, X., Li, P., et al. (2022) Effects of Inoculation with Acinetobacter on Fermentation of Cigar Tobacco Leaves. Frontiers in Microbiology, 13, Article ID: 911791. [Google Scholar] [CrossRef] [PubMed]
[42] Zhang, Q., Kong, G., Zhao, G., Liu, J., Jin, H., Li, Z., et al. (2023) Microbial and Enzymatic Changes in Cigar Tobacco Leaves during Air-Curing and Fermentation. Applied Microbiology and Biotechnology, 107, 5789-5801. [Google Scholar] [CrossRef] [PubMed]
[43] Jia, Y., Liu, Y., Hu, W., Cai, W., Zheng, Z., Luo, C., et al. (2023) Development of Candida Autochthonous Starter for Cigar Fermentation via Dissecting the Microbiome. Frontiers in Microbiology, 14, Article ID: 1138877. [Google Scholar] [CrossRef] [PubMed]
[44] 张蒙蒙. 单宁降解菌的筛选、鉴定及其在雪茄烟叶发酵中的应用研究[D]: [硕士学位论文]. 郑州: 河南农业大学, 2023.
[45] Wu, X., Zhu, P., Li, D., Zheng, T., Cai, W., Li, J., et al. (2021) Bioaugmentation of Bacillus amyloliquefaciensBacillus kochii Co-Cultivation to Improve Sensory Quality of Flue-Cured Tobacco. Archives of Microbiology, 203, 5723-5733. [Google Scholar] [CrossRef] [PubMed]
[46] 李志豪, 张鸽, 貊志杰, 等. 一株产木聚糖酶的蜡样芽孢杆菌对雪茄烟叶成分及发酵产物的影响[J]. 生物技术通报, 2022, 38(2): 105-112.
[47] 李宁, 汪长国, 曾代龙, 等. 蜡样芽孢杆菌(Bacillus cereus)筛选鉴定及在雪茄烟叶发酵中的应用研究[J]. 中国烟草学报, 2012, 18(2): 65-69.
[48] 毛亚浩, 张彤彤, 余君, 等. 混菌发酵对雪茄烟叶化学成分及香气品质的影响[J]. 湖北农业科学, 2023, 62(4): 140-145.